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环己烷、萘和柴油燃料会增加红球菌中氧化应激、CYP153、sodA 和 recA 基因的表达。

Cyclohexane, naphthalene, and diesel fuel increase oxidative stress, CYP153, sodA, and recA gene expression in Rhodococcus erythropolis.

机构信息

Southern Federal University, Rostov-on-Don, Russian Federation.

Institute for Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Jena, Germany.

出版信息

Microbiologyopen. 2019 Sep;8(9):e00855. doi: 10.1002/mbo3.855. Epub 2019 May 22.

DOI:10.1002/mbo3.855
PMID:31119875
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6816061/
Abstract

In this study, we compared the expression of CYP153, sodA, sodC, and recA genes and ROS generation in hydrocarbon-degrading Rhodococcus erythropolis in the presence of cyclohexane, naphthalene, and diesel fuel. The expression of cytochrome P450, sodA (encoding Fe/Mn superoxide dismutase), recA, and superoxide anion radical generation rate increased after the addition of all studied hydrocarbons. The peak of CYP153, sodA, and recA gene expression was registered in the presence of naphthalene. The same substrate upregulated the Cu/Zn superoxide dismutase gene, sodC. Cyclohexane generated the highest level of superoxide anion radical production. Hydrogen peroxide accumulated in the medium enriched with diesel fuel. Taken together, hydrocarbon biotransformation leads to oxidative stress and upregulation of antioxidant enzymes and CYP153 genes, and increases DNA reparation levels in R. erythropolis cells.

摘要

在这项研究中，我们比较了在环己烷、萘和柴油存在下，能够降解碳氢化合物的红色糖多孢菌中 CYP153、sodA、sodC 和 recA 基因的表达和 ROS 的生成。加入所有研究的碳氢化合物后，细胞色素 P450、sodA（编码 Fe/Mn 超氧化物歧化酶）、recA 和超氧阴离子自由基生成率增加。在萘存在下，CYP153、sodA 和 recA 基因的表达达到峰值。同一基质上调了 Cu/Zn 超氧化物歧化酶基因 sodC。环己烷产生的超氧阴离子自由基产量最高。在富含柴油的培养基中积累了过氧化氢。综上所述，碳氢化合物的生物转化导致了氧化应激和抗氧化酶和 CYP153 基因的上调，并增加了红色糖多孢菌细胞的 DNA 修复水平。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/801e/6816061/cac1bbd5a538/MBO3-8-e00855-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/801e/6816061/27e7bb302707/MBO3-8-e00855-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/801e/6816061/d768160ffaf6/MBO3-8-e00855-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/801e/6816061/7a7721cc6472/MBO3-8-e00855-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/801e/6816061/cac1bbd5a538/MBO3-8-e00855-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/801e/6816061/27e7bb302707/MBO3-8-e00855-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/801e/6816061/d768160ffaf6/MBO3-8-e00855-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/801e/6816061/7a7721cc6472/MBO3-8-e00855-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/801e/6816061/cac1bbd5a538/MBO3-8-e00855-g004.jpg

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