Department of Biological Sciences, University of Calgary, AB T2N 1N4, Canada.
Department of Biological Sciences, University of Calgary, AB T2N 1N4, Canada; Faculty of Science and Technology, Athabasca University, Athabasca, AB T9S 3A3, Canada.
Mar Pollut Bull. 2024 Nov;208:117052. doi: 10.1016/j.marpolbul.2024.117052. Epub 2024 Oct 1.
Permanently cold deep-sea sediments (2500-3500 m water depth) with and without indications of thermogenic hydrocarbon seepage were exposed to naphtha to examine the presence and potential of cold-adapted aerobic hydrocarbon-degrading microbial populations. Monitoring these microcosms for volatile hydrocarbons by GC-MS revealed sediments without in situ hydrocarbons responded more rapidly to naphtha amendment than hydrocarbon seep sediments overall, but seep sediments removed aromatic hydrocarbons benzene, toluene, ethylbenzene and xylene (BTEX) more readily. Naphtha-driven aerobic respiration was more evident in surface sediment (0-20 cmbsf) than deeper anoxic layers (>130 cmbsf) that responded less rapidly. In all cases, enrichment of Gammaproteobacteria included lineages of Oleispira, Pseudomonas, and Alteromonas known to be associated with marine oil spills. On the other hand, taxa known to be prevalent in situ and diagnostic for thermogenic hydrocarbon seepage in deep sea sediment, did not respond to naphtha amendment. This suggests a limited role for these prevalent seep-associated populations in the context of aerobic hydrocarbon biodegradation.
永久性寒冷深海沉积物（水深 2500-3500 米），无论是否存在热成因烃渗漏的迹象，都暴露在石脑油中，以检查冷适应好氧烃降解微生物种群的存在和潜力。通过 GC-MS 监测这些微宇宙中的挥发性烃，发现没有原位烃的沉积物对石脑油的响应总体上比烃渗漏沉积物更快，但渗漏沉积物更容易去除芳烃苯、甲苯、乙苯和二甲苯（BTEX）。石脑油驱动的需氧呼吸在表层沉积物（0-20 cmbsf）中比深层缺氧层（>130 cmbsf）更为明显，后者的反应速度较慢。在所有情况下，γ变形菌的富集都包括与海洋溢油有关的奥利氏菌、假单胞菌和交替单胞菌的谱系。另一方面，在原位普遍存在且对深海沉积物中热成因烃渗漏具有诊断意义的分类群，并未对石脑油的添加做出响应。这表明在好氧烃生物降解的情况下，这些普遍存在的与渗漏相关的种群的作用有限。
