State Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, People's Republic of China; Liaoning Key Laboratory for Mass Spectrometry Technology and Instrumentation, Dalian, 116023, People's Republic of China.
State Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, People's Republic of China; Liaoning Key Laboratory for Mass Spectrometry Technology and Instrumentation, Dalian, 116023, People's Republic of China; Northeastern University, College of Sciences, Research Center for Analytical Sciences, Department of Chemistry, Box 332, Shenyang, 110819, People's Republic of China.
Talanta. 2025 Jan 1;282:126975. doi: 10.1016/j.talanta.2024.126975. Epub 2024 Sep 29.
The escalating need for prompt and highly sensitive on-site detection of trace-level drugs is fueling the advancement of miniature, high-performance mass spectrometers and analytical methodologies. In this study, a miniature continuous atmospheric pressure interfaced ion trap mass spectrometer integrated with thermal desorption acetone-assisted photoionization (TD-CAPI-ITMS) was developed for highly sensitive detection of nonvolatile drugs in saliva and blood. By strategically extending the sampling time of the TD-CAPI-ITMS to cover the entire desorption process, a remarkable two-order-of-magnitude enhancement in the signal intensity of individual drugs was observed. Moreover, the simultaneous detection of drug mixtures with widely varying boiling points and saturation vapor pressures was accomplished. Optimization of the parameters yielded a limit of detection (LOD) for ketamine and 5F-EMB-PICA of 1 pg/μL accompanied by a robust stability, as evidenced by a relative standard deviation (RSD) of 5.30 %. Combined with straightforward liquid-liquid extraction, the sensitivity of drugs in saliva as low as 10 pg/μL was achieved, which met the requirements of Chinese national standard GA1333-2017. Owing to its exceptional sensitivity, the matrix effect present in blood samples was significantly alleviated through dilution, allowing for accurate monitoring of antibiotic concentrations. The results underscore the substantial potential of the TD-CAPI-ITMS for lab-free applications in drug-related forensic analysis, therapeutic drug monitoring, and pharmacokinetic studies.
微型连续大气压接口离子阱质谱仪与热脱附丙酮辅助光解（TD-CAPI-ITMS）联用，用于唾液和血液中痕量非挥发性药物的高灵敏检测。通过战略性地延长 TD-CAPI-ITMS 的采样时间以覆盖整个解吸过程，观察到各个药物的信号强度显著增强了两个数量级。此外，还实现了沸点和饱和蒸气压差异很大的药物混合物的同时检测。通过优化参数，实现了对氯胺酮和 5F-EMB-PICA 的检测限（LOD）为 1pg/μL，同时具有稳健的稳定性，相对标准偏差（RSD）为 5.30%。结合简单的液-液萃取，可实现唾液中药物的灵敏度低至 10pg/μL，满足中国国家标准 GA1333-2017 的要求。由于其出色的灵敏度，通过稀释显著减轻了血液样本中的基质效应，从而能够准确监测抗生素浓度。研究结果突出表明，TD-CAPI-ITMS 在无实验室应用于药物相关法医分析、治疗药物监测和药代动力学研究方面具有巨大潜力。
