Hagio Y
Nihon Sanka Fujinka Gakkai Zasshi. 1985 Nov;37(11):2369-75.
In order to investigate the antibacterial activity of amniotic fluid, the growth curves of several bacterial species in human amniotic fluid were observed by plate counting methods. The concentration of the bacteriolytic enzyme lysozyme in amniotic fluid was measured, and the relationship between lysozyme and antibacterial activity was discussed. The following results were obtained: Amniotic fluid had an antibacterial effect on B. subtilis, Staph. aureus and E. coli. When amniotic fluid was heated at 100 degrees C for 5 minutes, its antibacterial properties were lost or weakened. The lysozyme concentration in amniotic fluid increased gradually with gestational age, rising to 19.1 +/- 8.3 mu/ml at term. When lysozyme was added to amniotic fluid that had lost its antibacterial properties through exposure to heat, the antibacterial effect on B. subtilis was recovered. The growth of Staph. aureus in amniotic fluid exposed to heat was inhibited by the concomitant addition of lysozyme and aminobenzyl penicillin.
为了研究羊水的抗菌活性，采用平板计数法观察了几种细菌在人羊水中的生长曲线。测定了羊水中溶菌酶这种溶菌酶的浓度，并探讨了溶菌酶与抗菌活性之间的关系。得到以下结果：羊水对枯草芽孢杆菌、金黄色葡萄球菌和大肠杆菌有抗菌作用。当羊水在100℃加热5分钟时，其抗菌性能丧失或减弱。羊水中溶菌酶浓度随孕周逐渐升高，足月时升至19.1±8.3μg/ml。当将溶菌酶添加到因受热而失去抗菌性能的羊水中时，对枯草芽孢杆菌的抗菌作用得以恢复。同时添加溶菌酶和氨苄青霉素可抑制金黄色葡萄球菌在受热羊水中的生长。
