Department of Oral Biochemistry, School of Dentistry, Pusan National University, Yangsan, Republic of Korea; Periodontal Disease Signaling Network Research Center, School of Dentistry, Pusan National University, Yangsan, Republic of Korea; Institute for Future Earth, Pusan National University, Busan, Republic of Korea.
Interdisciplinary Program of Genomic Data Science, School of Medicine, Pusan National University, Yangsan, Republic of Korea.
J Dent. 2024 Dec;151:105366. doi: 10.1016/j.jdent.2024.105366. Epub 2024 Oct 1.
Peri-implantitis, a plaque-associated pathological condition, has been on the rise with the increasing prevalence of dental implants. Despite its similarities to periodontitis, peri‑implantitis is difficult to control completely and has high relapse rates. This has sparked interest in exploring the pathogenic differences between the two conditions.
This cross-sectional study involved 10 participants to concurrently examine periodontitis and peri‑implantitis within the same patients, thereby minimizing inter-individual variation. Gingival tissue samples were collected from each participant, comprising 10 periodontitis and 10 peri‑implantitis tissues, and RNAs were extracted. Using RNA sequencing and bioinformatics analysis, we investigated complex gene interactions, immune responses, and the role of the extracellular matrix in both conditions. We identified hub genes in each enhanced Protein-Protein Interaction network, providing crucial insights into these diseases' pathogenesis.
Our findings highlighted the potential involvement of activated fibroblasts in the pathogenesis of peri‑implantitis, identifying three marker genes (ACTA2, FAP, and PDGFRβ) overexpressed in peri‑implantitis, thus highlighting their potential as disease-specific biomarkers.
Our study uncovered a novel connection between peri‑implantitis and activated fibroblasts, examining specific markers and microbial differences between periodontitis and peri‑implantitis. These insights improve our understanding of peri‑implantitis pathogenesis, encouraging future research for better management and prevention strategies.
This study identifies key insights into the pathogenesis of peri‑implantitis compared to periodontitis. These findings promise to advance clinical approaches for better managing and preventing peri‑implantitis, addressing its complexities and high relapse rates effectively.
随着种植牙的普及，与菌斑相关的种植体周围炎呈上升趋势。尽管它与牙周炎有相似之处，但种植体周围炎很难完全控制，且复发率较高。这引发了人们对探索两种疾病发病机制差异的兴趣。
本横断面研究纳入了 10 名参与者，同期在同一患者中检查牙周炎和种植体周围炎，从而最大限度地减少个体间差异。从每位参与者中采集牙龈组织样本，包括 10 个牙周炎组织和 10 个种植体周围炎组织，并提取 RNA。我们利用 RNA 测序和生物信息学分析，研究了复杂的基因相互作用、免疫反应以及细胞外基质在两种情况下的作用。我们确定了每个增强型蛋白质-蛋白质相互作用网络中的枢纽基因，为这些疾病的发病机制提供了重要的见解。
我们的研究结果突出了激活的成纤维细胞在种植体周围炎发病机制中的潜在作用，确定了三个在种植体周围炎中过表达的标记基因（ACTA2、FAP 和 PDGFRβ），从而突出了它们作为疾病特异性生物标志物的潜力。
我们的研究揭示了种植体周围炎与激活的成纤维细胞之间的新联系，研究了牙周炎和种植体周围炎之间的特定标记物和微生物差异。这些见解提高了我们对种植体周围炎发病机制的理解，鼓励未来进行研究以制定更好的管理和预防策略。
与牙周炎相比，本研究确定了种植体周围炎发病机制的关键见解。这些发现有望推进针对种植体周围炎的临床方法，有效应对其复杂性和高复发率，改善对其的管理和预防。
