Primary Health Care Center, Department of Health, Medicine and Caring Sciences, Linköping University, Linköping, Sweden; Wallenberg Centre for Molecular Medicine, Linköping University, Linköping, Sweden.
Department of Radiation Physics, Department of Medical and Health Sciences, Linköping University, Linköping, Sweden.
Microvasc Res. 2025 Jan;157:104749. doi: 10.1016/j.mvr.2024.104749. Epub 2024 Sep 30.
Iontophoresis studies face challenges due to the unknown absolute drug dose delivered and the possible effect of the current used in drug delivery on the microvessels, known as current-induced vasodilation. This study aimed to investigate how various concentrations of acetylcholine (ACh), delivered through transdermal iontophoresis using repeated current pulses, impact the recovery profile of the microvascular response.
The study included fifteen healthy volunteers, and microvascular responses to five concentrations of iontophorised ACh (ranging from 0.0055 mM to 55 mM) and sterile water were assessed at six forearm skin sites using polarized reflectance spectroscopy. Iontophoresis at each concentration involved three consecutive pulses separated 8 recovery periods.
Current-induced responses were more pronounced for lower concentrations of ACh and for sterile water. With repeated pulses, lower concentrations of ACh exhibited a recovery profile more akin to higher concentrations.
Through repeated iontophoresis of ACh, microvascular responses exhibit variation based on the drug concentration and the number of pulses administered. These variations are likely attributed to changes in skin conductivity and permeability.
离子导入研究面临挑战，因为未知的绝对药物剂量以及电流对药物输送的可能影响，即电流诱导的血管扩张，会影响微血管。本研究旨在探讨通过经皮离子导入使用重复电流脉冲输送不同浓度的乙酰胆碱 (ACh) 如何影响微血管反应的恢复情况。
本研究包括 15 名健康志愿者，使用偏振反射光谱法在六个前臂皮肤部位评估离子导入的 ACh（浓度范围为 0.0055 mM 至 55 mM）和无菌水对微脉管反应的影响。每个浓度的离子导入涉及三个连续的脉冲，每个脉冲之间有 8 个恢复期。
较低浓度的 ACh 和无菌水的电流诱导反应更明显。随着重复脉冲的进行，较低浓度的 ACh 表现出更类似于较高浓度的恢复曲线。
通过重复离子导入 ACh，微脉管反应会根据药物浓度和给药脉冲数发生变化。这些变化可能归因于皮肤电导率和通透性的变化。
