通过乙酰胆碱离子电渗疗法评估内皮功能：电极间距和皮肤电阻的影响。
Assessment of endothelial function by acetylcholine iontophoresis: impact of inter-electrode distance and electrical cutaneous resistance.
作者信息
Puissant Cyril, Abraham Pierre, Durand Sylvain, Humeau-Heurtier Anne, Faure Sébastien, Leftheriotis Georges, Mahé Guillaume
机构信息
Laboratory of Vascular Investigations, University Hospital of Angers, France.
Laboratory of Vascular Investigations, University Hospital of Angers, France; L'UNAM University, University of Angers, Biologie Neurovasculaire et Mitochondriale Intégrée (BNMI), Unité mixte UMR CNRS 6214/INSERM U 1083, Medicine Faculty, Angers, France.
出版信息
Microvasc Res. 2014 May;93:114-8. doi: 10.1016/j.mvr.2014.04.001. Epub 2014 Apr 13.
OBJECTIVES
Endothelial function can be assessed by acetylcholine (ACh) iontophoresis with single current application. The effect of inter-electrode distance as well as electrical cutaneous resistance (ECR) on ACh dependent vasodilation has never been studied using single current application. The aims of this study are (i) to compare ACh-peak and ECR measured at different inter-electrode distances, (ii) to assess the relationship between ACh-peak and ECR, (iii) and to study the reproducibility of the ECR values.
METHODS
Fourteen healthy subjects were included. Using laser speckle contrast imaging, ACh-iontophoreses (0.1 mA, 30s) were performed on the forearm at a 7-day interval with an inter-electrode distance set at 5 cm. Two other inter-electrode distances were also evaluated: 10 cm and 15 cm. ECR was measured during each ACh-iontophoresis as well as the ACh-peak.
RESULTS
No statistical difference was found between the ACh-peak values obtained at 5 cm, 10 cm and 15 cm. ECRs were also not statistically different. An inverse relationship (r=-0.60) was found between the ACh-peak and ECR (p<0.05). The coefficient of variation of the inter-day reproducibility of the ECR values was 9.1% [6.5%-15.1%] with an intra-class-correlation coefficient of 0.93 [0.81-0.98].
CONCLUSION
Inter-electrode distance ranging from 5 cm to 15 cm changes neither the ACh-peak value nor the ECR value. ECR impacts ACh-peak values.
目的
内皮功能可通过单次施加电流的乙酰胆碱（ACh）离子电渗疗法进行评估。电极间距以及皮肤电阻（ECR）对ACh依赖性血管舒张的影响从未使用单次电流施加进行过研究。本研究的目的是：（i）比较在不同电极间距下测量的ACh峰值和ECR；（ii）评估ACh峰值与ECR之间的关系；（iii）研究ECR值的可重复性。
方法
纳入14名健康受试者。使用激光散斑对比成像，在前臂以7天的间隔进行ACh离子电渗疗法（0.1 mA，30秒），电极间距设定为5 cm。还评估了另外两个电极间距：10 cm和15 cm。在每次ACh离子电渗疗法期间测量ECR以及ACh峰值。
结果
在5 cm、10 cm和15 cm处获得的ACh峰值之间未发现统计学差异。ECR也无统计学差异。发现ACh峰值与ECR之间呈负相关（r = -0.60）（p<0.05）。ECR值日间可重复性的变异系数为9.1% [6.5%-15.1%]，组内相关系数为0.93 [0.81-0.98]。
结论
5 cm至15 cm的电极间距既不改变ACh峰值也不改变ECR值。ECR会影响ACh峰值。
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