Zhang Yunqi, Xiong Yu, Wu Xiaochun, Huang Maofang, Li Zhengjie, Zhao Tie, Peng Peng
Department of Pharmacy, Guangzhou Dermatology Hospital, Guangzhou, China.
Orthopedics Department, Guangdong Provincial Second Hospital of Traditional Chinese Medicine, Guangzhou, China.
J Cosmet Dermatol. 2024 Dec;23(12):3927-3935. doi: 10.1111/jocd.16606. Epub 2024 Oct 2.
Open skin wounds increase the risk of infections and can compromise health. Therefore, applying medications to promote healing at the injury site is crucial. In practice, direct drug delivery is often difficult to maintain for a long time due to rapid absorption or wiping off, which reduces the efficiency of wound healing. Consequently, the development of bioactive materials with both antibacterial and wound-healing properties is highly desirable.
This study synthesized liposomes loaded with glycyrrhizic acid (GA) and asiaticoside (AS) by film dispersion-ultrasonication method, which were then incorporated into a GelMA solution and cross-linked by ultraviolet light to form a bioactive composite hydrogel for wound dressings.
This hydrogel is conducive to the transport of nutrients and gas exchange. Compared with GelMA hydrogel (swelling rate 69.8% ± 5.7%), the swelling rate of GelMA/Lip@GA@AS is lower, at 52.1% ± 1.0%. GelMA/Lip@GA@AS also has better compression and rheological properties, and the in vitro biodegradability is not significantly different from that of the collagenase-treated group. In addition, the hydrogel polymer has a stable drug release rate, good biocompatibility, and an angiogenic promoting effect. In vitro experiments prove that, at concentrations of 0.5, 1, 2, and 3 mg/mL, GelMA/Lip@GA@AS can inhibit the growth of Staphylococcus aureus.
We synthesized GelMA/Lip@GA@AS hydrogel and found it possesses advantageous mechanical properties, rheology, and biodegradability. Experimental results in vitro showed that the bioactive hydrogel could efficiently release drugs, exhibit biocompatibility, and enhance angiogenesis and antimicrobial effects. These results suggest the promising application of GelMA/Lip@GA@AS hydrogel in wound-dressing materials.
开放性皮肤伤口会增加感染风险并损害健康。因此，在伤口部位应用促进愈合的药物至关重要。在实际应用中，由于药物快速吸收或被擦拭掉，直接给药往往难以长时间维持，这降低了伤口愈合的效率。因此，开发具有抗菌和伤口愈合特性的生物活性材料非常必要。
本研究通过薄膜分散 - 超声法合成了负载甘草酸（GA）和积雪草苷（AS）的脂质体，然后将其掺入甲基丙烯酰化明胶（GelMA）溶液中，并通过紫外光交联形成用于伤口敷料的生物活性复合水凝胶。
这种水凝胶有利于营养物质的运输和气体交换。与GelMA水凝胶（溶胀率69.8%±5.7%）相比，GelMA/Lip@GA@AS的溶胀率较低，为52.1%±1.0%。GelMA/Lip@GA@AS还具有更好的压缩和流变性能，其体外生物降解性与胶原酶处理组无显著差异。此外，水凝胶聚合物具有稳定的药物释放速率、良好的生物相容性和促血管生成作用。体外实验证明，在0.5、1、2和3mg/mL浓度下，GelMA/Lip@GA@AS可抑制金黄色葡萄球菌的生长。
我们合成了GelMA/Lip@GA@AS水凝胶，发现其具有优异的力学性能、流变学和生物降解性。体外实验结果表明，这种生物活性水凝胶能够有效释放药物，具有生物相容性，并增强血管生成和抗菌效果。这些结果表明GelMA/Lip@GA@AS水凝胶在伤口敷料材料中具有广阔的应用前景。
