School of the Environment and Safety Engineering, and Collaborative Innovation Center of Technology and Material of Water Treatment, Jiangsu University, Zhenjiang, 212013, P. R. China.
Fujian Key Laboratory of Inspection and Quarantine Technology Research, P. R. China.
Chem Commun (Camb). 2024 Oct 17;60(84):12229-12232. doi: 10.1039/d4cc03927k.
Residues of β-lactam antibiotics (β-LA) in the environment have posed a great threat to human health, while lacking a simple, effective, and universal sensing method. Herein, basic fuchsin and graphdiyne (GDY) were used as precursors to prepare the first reported acidity/basicity-sensitive GDY quantum dots (S-GDY QDs). We propose a novel fluorescence-sensing strategy for β-LA detection based on the ability of β-lactamases to catalyze β-LA to form carboxylic acid, which further induces a change in the acidity/basicity of the solution and causes a decrease in the fluorescence intensity of S-GDY QDs. Furthermore, a fluorescence test strip sensing platform integrated with a smartphone was established to achieve rapid, portable, and visual monitoring of β-LA. Using penicillin G as a model, a detection limit as low as 15.7 nM was achieved, showing important implications for β-LA detection.
β-内酰胺抗生素（β-LA）在环境中的残留对人类健康构成了极大威胁，而目前缺乏简单、有效、通用的传感方法。在此，我们使用碱性品红和二维石墨炔（GDY）作为前驱体，制备了首例报道的酸度/碱度敏感 GDY 量子点（S-GDY QDs）。我们提出了一种基于β-内酰胺酶将β-LA 催化生成羧酸的能力来检测β-LA 的新型荧光传感策略，这进一步导致溶液酸度/碱度发生变化，并使 S-GDY QDs 的荧光强度降低。此外，我们还建立了一个集成智能手机的荧光测试条传感平台，实现了对β-LA 的快速、便携和可视化监测。以青霉素 G 为模型，检测限低至 15.7 nM，这对β-LA 的检测具有重要意义。
