评估用于胰腺癌内镜超声引导下组织获取的针具的基因获取率和质量
Evaluation of Needles in Endoscopic Ultrasound-Guided Tissue Acquisition of Pancreatic Cancer for Genetic Yield and Quality.
作者信息
Tiong Jonathan, Nguyen Phi, Sritharan Mithra, Lundy Joanne, Shen Henry, Kumar Beena, Swan Michael, Jenkins Brendan, Croagh Daniel
机构信息
Department of Surgery, Monash Health, Melbourne, AUS.
Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Melbourne, AUS.
出版信息
Cureus. 2024 Sep 2;16(9):e68431. doi: 10.7759/cureus.68431. eCollection 2024 Sep.
BACKGROUND
Endoscopic ultrasound-guided fine needle biopsy (FNB) is the gold standard in tissue acquisition of pancreatic ductal adenocarcinoma (PDAC). There is a paucity of evidence of the impact of needle type or size on the genetic yield and quality.
METHODS
Patients 18 years and older with PDAC who underwent FNB were retrospectively identified from a single database from 2016 to 2021. Genetic quantity is measured in micrograms (µg) and quality defined by RNA or DNA integrity number (RIN and DIN). FNB needles examined were Acquire 22 gauge (Boston Scientific, Marlborough, MA, USA) and ProCore 22 and 20 gauges (Cook Medical, Bloomington, IN, USA).
RESULTS
Two hundred seventy-seven patients were identified. ProCore 20G needle procured higher RNA quantity (4125.8µg, IQR: 2003.8, 5954.8, p = 0.012) compared to ProCore 22G (2050µg IQR: 966.4, 3181.6) and Acquire 22G (2310.6µg, IQR: 1439.3, 4312). Median DNA quantity was 3340.5µg (Acquire 22G), 2610.4µg (ProCore 22G) and 3499.7µg (ProCore 20G) (p = 0.763). Median DIN was 7.3 (Acquire 22G and ProCore 22G) and 7.4 (ProCore 20G) (p = 0.449). Median RIN was 3.0 (Acquire 22G and ProCore 22G) and 2.7 (ProCore 20G) (p = 0.886).
CONCLUSION
ProCore 20G was associated with higher quantity of RNA. There were no differences in the quality acquired by different needles.
背景
内镜超声引导下细针穿刺活检（FNB）是胰腺导管腺癌（PDAC）组织获取的金标准。关于针型或尺寸对基因产量和质量影响的证据较少。
方法
从2016年至2021年的单一数据库中回顾性识别出18岁及以上接受FNB的PDAC患者。基因数量以微克（µg）为单位进行测量，质量由RNA或DNA完整性数值（RIN和DIN）定义。所检查的FNB针包括22号Acquire针（美国马萨诸塞州马尔伯勒市波士顿科学公司）以及22号和20号ProCore针（美国印第安纳州布卢明顿市库克医疗公司）。
结果
共识别出277例患者。与22号ProCore针（2050µg，四分位间距：966.4，3181.6）和22号Acquire针（2310.6µg，四分位间距：1439.3，4312）相比，20号ProCore针获取的RNA数量更高（4125.8µg，四分位间距：2003.8，5954.8，p = 0.012）。DNA数量中位数分别为3340.5µg（22号Acquire针）、2610.4µg（22号ProCore针）和3499.7µg（20号ProCore针）（p = 0.763）。DIN中位数分别为7.3（22号Acquire针和22号ProCore针）和7.4（20号ProCore针）（p = 0.449）。RIN中位数分别为3.0（22号Acquire针和22号ProCore针）和2.7（20号ProCore针）（p = 0.886）。
结论
20号ProCore针与更高的RNA数量相关。不同针获取的质量无差异。
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