Comparative study on chloroplast genome of species.

Tamaricaceae comprises about 120 species and has a long evolutionary history, Linn accounts for approximately 75% of the total species in this family. It is the most widely distributed and diverse genus in the family. They have important ecological significance for transforming deserts and improving climate conditions. However, is the most poorly classified genera among flowering plants owing to its large variability and high susceptibility to interspecific hybridization. In this study, the complete chloroplast genomes of three species and one draft chloroplast genome were obtained in this study. Combined with eight chloroplast genomes deposited in GenBank, complete chloroplast sequences of 12 species were used for further analysis. There are 176 non-SSR-related indels and 681 non-indel-related SSRs in the 12 chloroplast genomes. The mononucleotide SSRs are the most prevalent among all types of SSRs. The mVISTA results indicate high sequence similarities across the chloroplast genome, suggesting that the chloroplast genomes are highly conserved, except for sample (ENC850343). The IR regions and the coding regions are more conserved than the single-copy and noncoding regions. The , CAU, , and UAG regions are the most variable and have higher variability than those of the universal DNA markers. Finally, the first phylogenetic tree of Tamaricaceae was constructed which confirmed the monophyly of in Tamaricaceae. The first phylogenetic tree of was based on the complete chloroplast genome to date, the changes in branch length and support rate can potentially help us clarify the phylogenetic relationships of . All the obtained genetic resources will facilitate future studies in population genetics, species identification, and conservation biology of .