Liu Yanlei, Liu Kangjia, Dong Wenpan, Dong Shunping, Wang Yiheng, Xu Chao, Li Enze, Sun Jiahui
School of Landscape and Ecological Engineering Hebei University of Engineering Handan China.
School of Ecology and Nature Conservation Beijing Forestry University Beijing China.
Ecol Evol. 2025 Mar 27;15(4):e71141. doi: 10.1002/ece3.71141. eCollection 2025 Apr.
The Hamamelidaceae is significant for its contributions to construction, furniture making, and ornamental use, including 26 genera and 119 species. However, complete chloroplast genome sequences of Hamamelidaceae species have been reported less frequently. In this study, five species were newly sequenced, and seven others available complete chloroplast genomes were added to compare the chloroplast genome evolution in Hamamelidaceae at the subfamily level. The results indicated that the chloroplast genome size ranged from 158,116 to 159,941 bp, encoding 79 to 81 protein-coding genes, four ribosomal RNA genes, and 30 to 31 transfer RNA genes. A robust phylogenetic tree of Hamamelidaceae was obtained using complete chloroplast genomes, supporting that all Hamamelidaceae species formed a monophyletic group and divided into four subfamilies. Exbucklandioideae was the first diverged group within Hamamelidaceae, followed by Mytilarioideae, Disanthoideae, and Hamamelidoideae, which formed a clade. Furthermore, three new potential DNA barcodes were provided: , , and . This study confirms that the complete chloroplast genome data provide a more accurate and confident resolution of the phylogenetic relationships within the Hamamelidaceae. These new genomic data not only enhance the understanding of genome evolution but also provide a better understanding of the phylogenetic relationships of Hamamelidaceae.
金缕梅科因其在建筑、家具制造和观赏用途方面的贡献而具有重要意义，包括26个属和119个物种。然而，金缕梅科物种完整的叶绿体基因组序列报道较少。在本研究中，新测序了5个物种，并添加了另外7个已有的完整叶绿体基因组，以比较金缕梅科亚科水平上的叶绿体基因组进化。结果表明，叶绿体基因组大小在158,116至159,941bp之间，编码79至81个蛋白质编码基因、4个核糖体RNA基因和30至31个转运RNA基因。利用完整的叶绿体基因组获得了一个可靠的金缕梅科系统发育树，支持所有金缕梅科物种形成一个单系群，并分为四个亚科。马蹄荷亚科是金缕梅科中第一个分化出来的类群，其次是壳菜果亚科、双花木亚科和金缕梅亚科，它们形成一个分支。此外，还提供了三个新的潜在DNA条形码： ， 和 。本研究证实，完整的叶绿体基因组数据为金缕梅科内的系统发育关系提供了更准确和可靠的解析。这些新的基因组数据不仅增强了对基因组进化的理解，也为更好地理解金缕梅科的系统发育关系提供了帮助。
