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.提取物的植物化学分析及体外生物活性评估

Phytochemical analysis and in vitro biological activity assessment of extracts from .

作者信息

T Taskın, M Oksuz, E Rayaman, M Ermanoglu, D Taskın, A G Acar, Ö Kılıç, E Çalışkan Salihi, B Yılmaz Nur, Hk Elçioğlu

机构信息

Department of Pharmacognosy, Faculty of Pharmacy, Marmara University, Istanbul, Turkey.

Marmara Pharmacy Drug and Innovative Product Development Unit, Faculty of Pharmacy, Marmara University, Istanbul, Turkey.

出版信息

Int J Environ Health Res. 2025 Jul;35(7):1833-1845. doi: 10.1080/09603123.2024.2409829. Epub 2024 Oct 3.

Abstract

In this study, the antioxidant capacity, the effect on anti-acetylcholinesterase and anti-urease enzyme inhibition, and kidney stone-reducing effects of different extracts from . In addition, the phytochemical content of the bioactive methanol extract was analyzed by HPLC-DAD. The methanol extract showed strong DPPH (IC:0.036 mg/mL) radical scavenging, Cu (II) ion (6.535 mmTroloxE) and Fe (6.476 FeSO) ion reducing antioxidant activity compared to other extracts. The methanol extract contained the highest amount of phenolic (400 mgGAE) and flavonoid (2.519 QuE) compounds. Moreover, the methanol extract had significant anticholinesterase (78.935%), anti-urease (71.014%) and calcium oxalate anti-crystallization (18.22%, 37.427%) activities. The findings show that all extracts exhibited varying amounts of antibacterial activity against . The bioactive methanol extract contained rosmarinic acid (31.35 µg), quercetin (12.99 µg) and chlorogenic acid (3.55 µg). The analyzed compounds are thought to contribute significantly to the pharmacological effect of the methanol extract.

摘要

在本研究中,[植物名称]不同提取物的抗氧化能力、对乙酰胆碱酯酶抑制和脲酶抑制的作用以及减少肾结石的效果。此外,通过高效液相色谱 - 二极管阵列检测器(HPLC - DAD)分析了生物活性甲醇提取物的植物化学成分。与其他提取物相比,甲醇提取物表现出较强的二苯基苦味酰基自由基(DPPH,IC:0.036 mg/mL)清除能力、铜(II)离子(6.535 mm Trolox E)和铁(6.476 FeSO)离子还原抗氧化活性。甲醇提取物中酚类(400 mg GAE)和黄酮类(2.519 QuE)化合物含量最高。此外,甲醇提取物具有显著的抗胆碱酯酶(78.935%)、抗脲酶(71.014%)和草酸钙抗结晶(18.22%,37.427%)活性。研究结果表明,所有提取物对[细菌名称]均表现出不同程度的抗菌活性。生物活性甲醇提取物含有迷迭香酸(31.35 µg)、槲皮素(12.99 µg)和绿原酸(3.55 µg)。据认为,所分析的这些化合物对甲醇提取物的药理作用有显著贡献。

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