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Choisy 的不同解剖部位的植物化学分析、抗氧化潜力和抗菌活性

Phytochemical Analysis, Antioxidant Potential and Antibacterial Activities of Different Anatomical Parts of Choisy.

机构信息

Department of Chemistry, Tri-Chandra Campus, Tribhuvan University, Kirtipur, Nepal.

Natural Products Laboratory, Research Centre for Applied Science and Technology, Tribhuvan University, Kathmandu 44600, Nepal.

出版信息

ScientificWorldJournal. 2024 May 25;2024:8128813. doi: 10.1155/2024/8128813. eCollection 2024.

Abstract

The genus comprises a large number of species. The flower, leaf, stem, and root of the species are widely used in traditional medicine in different cultures. Many species have been well investigated phytochemically and pharmacologically. However, only a few reports are available on the native to Nepal. The present study aims to evaluate the phytochemical composition of different extracts, qualitative analysis of methanol extract of the flower and leaf using thin-layer chromatography (TLC), and the antioxidant properties of components by the TLC-DPPH assay. The phenolic and flavonoid contents were estimated in different extracts of the leaf and stem, and their antioxidant and antibacterial activities were evaluated. In the phytochemical screening, phenolics and flavonoids were present in ethyl acetate, methanol, and 50% aq methanol extracts of both the leaf and stem. In TLC analysis, the methanol extract of flowers showed the presence of 11 compounds and the leaf extract showed the presence of 8 compounds. Both extracts contained chlorogenic acid and mangiferin. Hyperoside and quercetin were present only in the flower extract. In the TLC-DPPH assay, almost all of the flower extracts and 5 compounds of the leaf extract showed radical scavenging potential. Estimation of phenolics and flavonoids showed that all the leaf extracts showed higher amounts of phenolics and flavonoids than stem extracts. Among leaf extracts, greater amounts of phenolics were detected in 50% aqueous methanol extract (261.25 ± 1.66 GAE/g extract) and greater amounts of flavonoids were detected in methanol extract (232.60 ± 10.52 CE/g extract). Among stem extracts, greater amounts of flavonoids were detected in the methanol extract (155.12 ± 4.30 CE/g extract). In the DPPH radical scavenging assay, the methanol extract of the leaf showed IC 60.85 ± 2.67 g/ml and 50% aq. methanol extract of the leaf showed IC 63.09 ± 2.98 g/ml. The methanol extract of the stem showed IC 89.39 ± 3.23 g/ml, whereas ethyl acetate and 50% aq. methanol extract showed IC > 100 g/ml. In the antibacterial assay, the methanol extract of the leaf showed the inhibition zone of 12-13 mm and the stem extract showed the inhibition zone of 7-11 mm against , , and , whereas both extracts were inactive against . The findings of this study support the traditional use of this plant in Nepal for the treatment of diseases associated with bacterial infections. The present study revealed that the underutilized anatomical parts of could be the source of various bioactive phytochemicals like other species.

摘要

该属包含大量物种。不同文化中, 种的花、叶、茎和根被广泛用于传统医学。许多 种已被很好地进行了植物化学和药理学研究。然而,仅有少数报道涉及尼泊尔的 种。本研究旨在评估不同提取物的植物化学成分,使用薄层层析 (TLC) 对花和叶的甲醇提取物进行定性分析,并通过 TLC-DPPH 测定法评估成分的抗氧化特性。在叶和茎的不同提取物中估计了酚类和类黄酮的含量,并评估了它们的抗氧化和抗菌活性。在植物化学筛选中,叶和茎的乙酸乙酯、甲醇和 50% 甲醇提取物中均含有酚类和类黄酮。在 TLC 分析中,花的甲醇提取物显示存在 11 种化合物,叶的提取物显示存在 8 种化合物。两种提取物均含有绿原酸和芒果苷。根皮苷和槲皮素仅存在于花提取物中。在 TLC-DPPH 测定中,花的几乎所有提取物和叶的 5 种提取物均显示出自由基清除潜力。酚类和类黄酮的估计表明,所有叶提取物的酚类和类黄酮含量均高于茎提取物。在叶提取物中,50% 甲醇水提取物中的酚类含量更高(261.25 ± 1.66 GAE/g 提取物),而甲醇提取物中的类黄酮含量更高(232.60 ± 10.52 CE/g 提取物)。在茎提取物中,甲醇提取物中的类黄酮含量更高(155.12 ± 4.30 CE/g 提取物)。在 DPPH 自由基清除测定中,叶的甲醇提取物的 IC 60.85 ± 2.67 g/ml,叶的 50% 水甲醇提取物的 IC 63.09 ± 2.98 g/ml。茎的甲醇提取物的 IC 89.39 ± 3.23 g/ml,而乙酸乙酯和 50% 水甲醇提取物的 IC > 100 g/ml。在抗菌测定中,叶的甲醇提取物对 、 、 和 显示出 12-13mm 的抑菌圈,而茎提取物对 、 、 和 显示出 7-11mm 的抑菌圈,而两种提取物对 均无活性。本研究的结果支持尼泊尔传统上使用该植物治疗与细菌感染相关的疾病。本研究表明, 未充分利用的解剖部位可能是其他 种的各种生物活性植物化学物质的来源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2776/11144067/5bdd3d7b1cba/TSWJ2024-8128813.001.jpg

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