Liu Huai, Gu Wenjie, Lu Yusheng, Ding Lili, Guo Yating, Zou Geng, Wu Weiqing, Zheng Diyuan, Liu Chong, Wang Chenyang, Cao Yangrong, Li Jinquan
Key Laboratory of Plant Nutrition and Fertilizer in South Region, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Nutrient Cycling and Farmland Conservation, Guangdong Engineering Research Center of Soil Microbes and Cultivated Land Conservation, Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Key Laboratory of Environment Correlative Dietology, College of Biomedicine and Health, College of Food Science and Technology, College of Life Science and Technology, College of Veterinary Medicine, Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Wuhan 430070, China.
J Agric Food Chem. 2024 Dec 18;72(50):28005-28018. doi: 10.1021/acs.jafc.4c03799. Epub 2024 Oct 3.
Phage therapy has the potential to alleviate plant bacterial wilt. However, the knowledge gap concerning the phage-agrochemical interaction impedes the broader application of phages in agriculture. This study characterized a phage isolate and investigated its interactions with agrochemicals. A novel species within the genus was proposed, serving phage LPRS20 as a type phage with a broad lytic range and significant antibacterial activity against strains infecting tobacco, chili, or tomato. Sensory evaluation of the morphology of tobacco leaves suggested that phage application resulted in negligible harm to plants. Investigations into phage-agrochemical interactions revealed synergisms when LPRS20 was delivered 4 h before thiodiazole-copper as well as LPRS20 in combination with low-concentration berberine. Overall, our findings reveal that phage LPRS20 represents a novel, effective, and eco-friendly biocontrol agent against tobacco bacterial wilt and and contributes to the potential integration of phages and agrochemicals for controlling soil-borne pathogens.
噬菌体疗法有减轻植物青枯病的潜力。然而，关于噬菌体与农用化学品相互作用的知识空白阻碍了噬菌体在农业中的更广泛应用。本研究对一种噬菌体分离株进行了表征，并研究了它与农用化学品的相互作用。在该属内提出了一个新物种，将噬菌体LPRS20作为具有广泛裂解范围且对感染烟草、辣椒或番茄的菌株具有显著抗菌活性的模式噬菌体。对烟草叶片形态的感官评价表明，施用噬菌体对植物造成的损害可忽略不计。对噬菌体与农用化学品相互作用的研究表明，当LPRS20在噻菌铜之前4小时施用时以及LPRS20与低浓度黄连素联合使用时存在协同作用。总体而言，我们的研究结果表明，噬菌体LPRS20是一种新型、有效且环保的防治烟草青枯病的生物防治剂，并有助于噬菌体和农用化学品在控制土传病原体方面的潜在整合。
