Glasby M A
Q J Exp Physiol. 1985 Oct;70(4):585-9. doi: 10.1113/expphysiol.1985.sp002945.
It has been suggested that since mannitol is excreted in the bile it may enter the liver cell and hence over-estimate extracellular fluid volume if used as a marker. Boluses of D-[14C]mannitol were injected into anaesthetized, nephrectomized dogs. After equilibration, liver biopsy samples were taken. Total liver water was estimated gravimetrically and compared with extracellular fluid volume estimated from the biopsy samples using beta-counting. There was no significant difference between these two estimations. However D-[14C]mannitol and [14C]inulin carboxylic acid spaces for liver were significantly different. This suggests that mannitol penetrates liver cells. A correction factor is derived to enable the technique to be used more accurately in 'whole-body' extracellular fluid volume estimation.
有人提出，由于甘露醇经胆汁排泄，它可能进入肝细胞，因此如果用作标志物，可能会高估细胞外液容量。将大剂量的D-[14C]甘露醇注入麻醉的、肾切除的狗体内。平衡后，采集肝脏活检样本。通过重量法估算肝脏总水量，并与使用β计数法从活检样本估算的细胞外液容量进行比较。这两种估算之间没有显著差异。然而，肝脏的D-[14C]甘露醇和[14C]菊粉羧酸空间有显著差异。这表明甘露醇可穿透肝细胞。推导了一个校正因子，以使该技术能够更准确地用于“全身”细胞外液容量估算。
