Pretus H A, Ignarro L J, Ensley H E, Feigen L P
Prostaglandins. 1985 Oct;30(4):591-8. doi: 10.1016/0090-6980(85)90023-1.
To determine whether agents which inhibit cytochrome P-450 enzymes also inhibit lipoxygenase, the effects of metyrapone and SKF 525-A were assessed on soybean lipoxygenase using a spectrophotometric technique which allows for measurement of both the rate and magnitude of product formation. Both SKF 525-A and metyrapone inhibited the rate of product formation and the final amount of product formed in 5 min incubations SKF 525-A was 5 to 5 times more potent than metyrapone, with the IC50 for SKF 525-A 40 microM and for metyrapone between 150 and 200 microM as determined by the total product formation in 5 minutes. Analysis of the reduced product by HPLC confirmed that the substances monitored were those generated by the 15-lipoxygenase enzyme.
为了确定抑制细胞色素P - 450酶的试剂是否也抑制脂氧合酶，使用一种分光光度技术评估了甲吡酮和SKF 525 - A对大豆脂氧合酶的影响，该技术能够测量产物形成的速率和量。SKF 525 - A和甲吡酮均抑制了产物形成的速率以及在5分钟孵育中形成的最终产物量。SKF 525 - A的效力比甲吡酮强5至5倍，根据5分钟内的总产物形成情况测定，SKF 525 - A的IC50为40微摩尔，甲吡酮的IC50在150至200微摩尔之间。通过高效液相色谱法对还原产物的分析证实，所监测的物质是由15 - 脂氧合酶产生的那些物质。
