细胞悬液和冰冻组织切片中B细胞非霍奇金淋巴瘤的表面免疫球蛋白限制

Surface immunoglobulin restriction in B-non Hodgkin's lymphomas in cell suspension and on frozen tissue sections.

作者信息

Kluin P M, de Weger R A, Schuurman H J, Peters P P, Spies P I, Bast B J, van Unnik J A, de Gast G C

出版信息

Scand J Haematol. 1985 Oct;35(4):399-407. doi: 10.1111/j.1600-0609.1985.tb02263.x.

DOI:10.1111/j.1600-0609.1985.tb02263.x

Abstract

The diagnostic relevance of different tests for detection of surface immunoglobulin on tumour cells of B-type non-Hodgkin's lymphomas (B-NHL) was investigated by comparison of the direct antiglobulin rosetting reaction (DARR) in suspension with two-colour direct immunofluorescence (DIF) on frozen tissue sections. In benign lymph nodes (n = 27) the kappa/lambda ratio by DARR test ranged from 0.9 to 2.8. Tested by suspension and frozen tissue analysis, light chain restriction was found in 24 and 27 of 31 cases of B-NHL, respectively. Heavy chain restriction was found in half of the cases (14 of 26) studied in suspension and in almost all (28 of 31) tested on sections. In 9 cases DARR tests showed restriction of more than one Ig class on tumour cells, which was infrequent (2 of 28) in frozen section analysis. Although both tests appeared valuable for routine diagnostic purposes, we found the DIF analysis on tissue sections somewhat more discriminative, especially in detection of heavy chain restriction in B-NHL.

摘要

通过比较悬浮液中的直接抗球蛋白玫瑰花结反应（DARR）与冷冻组织切片上的双色直接免疫荧光（DIF），研究了不同检测方法对B型非霍奇金淋巴瘤（B-NHL）肿瘤细胞表面免疫球蛋白检测的诊断相关性。在良性淋巴结（n = 27）中，DARR试验的κ/λ比值范围为0.9至2.8。通过悬浮液和冷冻组织分析检测，在31例B-NHL病例中，分别有24例和27例发现轻链限制。在悬浮液中研究的一半病例（26例中的14例）以及在切片上检测的几乎所有病例（31例中的28例）中发现重链限制。在9例病例中，DARR试验显示肿瘤细胞上不止一种Ig类别的限制，这在冷冻切片分析中很少见（28例中的2例）。尽管两种检测方法对常规诊断都有价值，但我们发现组织切片上的DIF分析更具鉴别力，尤其是在检测B-NHL中的重链限制方面。