Scher I, Sharrow S O, Wistar R, Asofsky R, Paul W E
J Exp Med. 1976 Aug 1;144(2):494-506. doi: 10.1084/jem.144.2.494.
The density of total Ig and of IgM, IgG1, IgG2, and IgA on the surface of adult murine splenic B lymphocytes was measured using the technique of rapid flow microfluorometry. In addition, the density of total surface Ig and of IgM on B lymphocytes derived from adult bone marrow, lymph nodes, and Peyer's patches, and from neonatal spleen was determined. Adult spleen and lymph node B lymphocytes are characterized by the presence of a large population of cells with a low-to-intermediate density of total surface Ig, which is seen as a peak in the fluorescence profiles when these cells are labeled with fluorescein-conjugated (F1) anti-Ig. This peak is not detected when neonatal spleen or adult bone marrow are examined; the development of this peak among spleen cells occurs during the first 4 wk of life. Although the characteristic fluorescence intensity peak is not seen when adult spleen cells are labeled with Fl anti-mu, changes in the density of surface IgM do occur during the first few weeks of life and are detected as a decrease in the frequency of cells which have relatively large amounts of surface IgM. The differences seen in the fluorescence patterns of adult spleen cells labeled with Fl anti-Ig and Fl anti-mu may be due to the appearance of IgD on the surface of mature splenic B lymphocytes. This is supported by the similarity of the fluorescence profiles of adult bone marrow cells stained with Fl anti-Ig and Fl anti-mu, as the latter population of cells is reported to lack surface IgD.
采用快速流动微荧光测定技术，测量成年小鼠脾脏B淋巴细胞表面总免疫球蛋白（Ig）以及IgM、IgG1、IgG2和IgA的密度。此外，还测定了来自成年骨髓、淋巴结、派伊尔结以及新生脾脏的B淋巴细胞表面总Ig和IgM的密度。成年脾脏和淋巴结B淋巴细胞的特征是，存在大量总表面Ig密度低至中等的细胞，当用荧光素偶联（F1）抗Ig标记这些细胞时，这在荧光图谱中表现为一个峰值。检查新生脾脏或成年骨髓时未检测到这个峰值；脾脏细胞中这个峰值的出现发生在出生后的前4周。虽然用F1抗μ标记成年脾脏细胞时未见到特征性荧光强度峰值，但表面IgM密度在出生后的头几周确实发生了变化，表现为表面IgM相对较多的细胞频率降低。用F1抗Ig和F1抗μ标记成年脾脏细胞时荧光模式的差异，可能是由于成熟脾脏B淋巴细胞表面出现了IgD。成年骨髓细胞用F1抗Ig和F1抗μ染色时荧光图谱的相似性支持了这一点，因为据报道后一组细胞缺乏表面IgD。
