Recent development of gold nanochips in biosensing and biodiagnosis sensibilization strategies in vitro based on SPR, SERS and FRET optical properties.

Gold nanomaterials have become attractive nanomaterials for biomedical research due to their unique physical and chemical properties, and nanochips are designed to manufacture high-quality substrates for loading gold nanoparticles (GNPs) to achieve specific and selective detection. By utilizing multiple optical properties of different gold nanostructures, the sensitivity, specificity, speed, contrast, resolution, and other performance of biosensing and biological diagnosis can be significantly improved. This paper summarized the sensitivity enhancement strategies of optical biosensing techniques based on the three main optical properties of gold nanomaterials: surface plasmon resonance (SPR), surface-enhanced Raman scattering (SERS) and fluorescence resonance energy transfer (FRET). The aim is to comprehensively review the development direction of in vitro diagnostics (IVDs) from two aspects: detection strategies and modification of gold nanomaterials. In addition, some opportunities and challenges that gold-based IVDs may encounter at present or in the future are also mentioned in this paper. In summary, this paper can enlighten readers with feasible strategies for manufacturing potential gold-based nanobiosensors.