Dembélé-Duchesne M J, Laghchim Lahlou A, Thaler-Dao H, Crastes de Paulet A
Thromb Haemost. 1985 Aug 30;54(2):431-7.
Human placental cytosol inhibits platelet aggregation induced by high doses of collagen. The aim of this study was to investigate whether this anti-aggregating activity was caused only by the presence of various activities already described in the placenta (an ADP-consuming enzyme, a fatty acid cyclooxygenase inhibitor, and a thromboxane synthetase inhibitor) or whether another factor was present. Heating the cytosol at 50 degrees C for 6 min destroyed the inhibitor of collagen-induced aggregation. ADPase and the AA pathway inhibitors were not modified by this treatment. We therefore show the presence of an additional anti-aggregating factor: it is destroyed by heating at 50 degrees C. We also tested for the presence of an inhibitor of AA release in the placental cytosol using three different methods (rabbit platelets in PRP, washed rabbit platelets, and NRK fibroblasts) but no inhibition could be evidenced. We conclude that this new anti-aggregating factor, which is probably a protein, acts neither through AA release inhibition nor AA cascade inhibition.
人胎盘胞质溶胶可抑制高剂量胶原蛋白诱导的血小板聚集。本研究的目的是调查这种抗聚集活性是否仅由胎盘中已描述的各种活性物质（一种消耗ADP的酶、一种脂肪酸环氧化酶抑制剂和一种血栓素合成酶抑制剂）的存在引起，还是存在其他因素。将胞质溶胶在50℃加热6分钟会破坏胶原蛋白诱导聚集的抑制剂。这种处理不会改变ADP酶和花生四烯酸（AA）途径抑制剂。因此，我们证明存在一种额外的抗聚集因子：它在50℃加热时会被破坏。我们还使用三种不同方法（富血小板血浆中的兔血小板、洗涤后的兔血小板和NRK成纤维细胞）检测了胎盘胞质溶胶中是否存在AA释放抑制剂，但未发现抑制作用。我们得出结论，这种新的抗聚集因子可能是一种蛋白质，它既不通过抑制AA释放起作用，也不通过抑制AA级联反应起作用。
