Iioka H, Hisanaga H, Moriyama I, Akasaki M, Nabuchi K, Katakami Y, Katoh Y, Ichijo M
Department of Obstetrics and Gynecology, Nara Medical University.
Nihon Sanka Fujinka Gakkai Zasshi. 1989 Dec;41(12):1916-20.
To clarify the role of placental brush border in the regulation of placental microcirculation, we investigated the platelet aggregation inhibition activity of placental brush border membrane vesicles (BBMV) and obtained the following results. 1. There was very strong platelet aggregation inhibiting activity in placental BBMV. In the 20-40 micrograms/ml protein concentration, the BBMV almost completely inhibited the platelet aggregation induced by ADP, arachidonic acid and collagen, and in the 100-150 micrograms/ml protein concentration, the BBMV almost completely inhibited the platelet aggregation induced by ristocetin. 2. There existed a very strong ADP degrading activity (ADPase activity) in the placental BBMV. The platelet aggregation activity of ADP was completely lost when ADP was preincubated with the placental BBMV. 3. The placental BBMV inhibited platelet malondialdehyde (MDA) production. With the 20 micrograms/ml and 100 micrograms/ml protein concentrations of placental BBMV, 22% and 65% of platelet MDA production was inhibited respectively.
为阐明胎盘刷状缘在胎盘微循环调节中的作用，我们研究了胎盘刷状缘膜囊泡（BBMV）的血小板聚集抑制活性，并获得以下结果。1. 胎盘BBMV具有非常强的血小板聚集抑制活性。在蛋白质浓度为20 - 40微克/毫升时，BBMV几乎完全抑制了由二磷酸腺苷（ADP）、花生四烯酸和胶原蛋白诱导的血小板聚集；在蛋白质浓度为100 - 150微克/毫升时，BBMV几乎完全抑制了由瑞斯托菌素诱导的血小板聚集。2. 胎盘BBMV中存在非常强的ADP降解活性（ADP酶活性）。当ADP与胎盘BBMV预孵育时，ADP的血小板聚集活性完全丧失。3. 胎盘BBMV抑制血小板丙二醛（MDA）的产生。在胎盘BBMV蛋白质浓度为20微克/毫升和100微克/毫升时，分别抑制了22%和65%的血小板MDA产生。
