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[用于检测肉和蛋中抗生素残留的电泳法]

[Electrophoretic method of demonstrating antibiotic residues in meat and eggs].

作者信息

Ionova I, Petkov R

出版信息

Vet Med Nauki. 1985;22(9):78-84.

PMID:3936255
Abstract

Studies were carried out on Smither and Vanghan's electrophoretic method with the use of high voltage. The method was found to be readily reproducible and to have good sensitivity. It could be successfully used in the demonstration and identification of residual amounts of antibiotics in meat and eggs. It was also established that with the use of agarose of pH 6.0 amino glycosides, beta-lactams, flavomycin, spectinomycin, and monensin showed higher migration capacity. The same value of pH was contributive to increasing the migration distances with macrolites, zincbacitracin, and tetracyclines. It was demonstrated that the use of electric current at 50 MA made it more favourable to separate and identify antibiotics. B. subtilis BGA was found to be more sensitive than B. mycoides v. cereus ATCC 11778 with regard to the antibiotics used and it could be made use of in the demonstration of minimal amounts.

摘要

采用高压对史密斯和万汉的电泳方法进行了研究。结果发现该方法易于重现且灵敏度良好。它可成功用于肉类和蛋类中残留抗生素的检测与鉴定。还证实,使用pH值为6.0的琼脂糖时,氨基糖苷类、β-内酰胺类、黄霉素、壮观霉素和莫能菌素显示出更高的迁移能力。相同的pH值有助于增加大环内酯类、杆菌肽锌和四环素类的迁移距离。结果表明,使用50毫安的电流更有利于抗生素的分离和鉴定。就所用抗生素而言,枯草芽孢杆菌BGA比蜡样芽孢杆菌ATCC 11778更敏感,可用于痕量检测。

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