Koenen-Dierick K, De Beer J O
Ministry of Public Health, Scientific Institute of Public Health-Louis Pasteur, Department of Pharmacobromatology, Brussels, Belgium.
Food Addit Contam. 1998 Jul;15(5):528-34. doi: 10.1080/02652039809374677.
Experimental design was applied to an agar-diffusion inhibition test for the screening of residues of veterinary antimicrobial drugs in slaughter animals. The effects and interactions of four independent parameters were studied: the dextrose content of the culture medium, the trimethoprim concentration in the culture medium, the thickness of the agar-layer and the preincubation time. The effects on the inhibition zones of sulphadimidine, oxytetracycline, streptomycin and tylosin, substances of four different antibiotic groups were measured. An optimized multi-residue test was established with the parameter values that yielded the best sensitivity for the four substances.
实验设计应用于琼脂扩散抑制试验，用于筛选屠宰动物中兽药抗菌药物残留。研究了四个独立参数的影响及相互作用：培养基中的葡萄糖含量、培养基中的甲氧苄啶浓度、琼脂层厚度和预孵育时间。测定了四种不同抗生素组的物质磺胺二甲嘧啶、土霉素、链霉素和泰乐菌素对抑菌圈的影响。利用对这四种物质产生最佳灵敏度的参数值建立了优化的多残留检测方法。
