Shirmohammadi Mehrnaz, Kianersi Farahnaz, Shiry Nima, Burgos-Aceves Mario Alberto, Faggio Caterina
South of Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Ahvaz, Iran.
Persian Gulf and Oman Sea Ecological Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas, Iran; Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Sci Total Environ. 2024 Dec 1;954:176661. doi: 10.1016/j.scitotenv.2024.176661. Epub 2024 Oct 1.
This study investigates the combined toxicity of microplastics (MPs) and florfenicol (FLO) on biotransformation enzymes and oxidative stress biomarkers in the liver and kidney of yellowfin seabream (Acanthopagrus latus). Fish were fed 15 mg kg of FLO and 100 or 500 mg kg of MPs for 10 days. Biomarkers, including ethoxyresorufin-O-deethylase, glutathione-S-transferase, superoxide dismutase, catalase, glutathione peroxidase, malondialdehyde (MDA), and protein carbonylation (PC), were measured in both organs at 1, 7, and 14 days post-exposure. FLO levels peaked on day 1 and declined after that. Liver biomarkers were more responsive to pollutants, with the combined exposure of FLO and MPs leading to more pronounced toxicity. By day 14, only the FLO group showed a return to baseline biomarker levels, while MDA and PC levels remained elevated in MPs and co-exposed groups. These findings highlight the importance of considering the interactive effects of multiple pollutants in addressing marine environmental stressors.
本研究调查了微塑料（MPs）和氟苯尼考（FLO）对黄鳍鲷（Acanthopagrus latus）肝脏和肾脏中生物转化酶及氧化应激生物标志物的联合毒性。给鱼投喂15毫克/千克的FLO和100或500毫克/千克的MPs，持续10天。在暴露后第1、7和14天，对两个器官中的生物标志物进行测量，包括乙氧基异吩恶唑酮-O-脱乙基酶、谷胱甘肽-S-转移酶、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、丙二醛（MDA）和蛋白质羰基化（PC）。FLO水平在第1天达到峰值，之后下降。肝脏生物标志物对污染物的反应更敏感，FLO和MPs的联合暴露导致更明显的毒性。到第14天，只有FLO组的生物标志物水平恢复到基线，而MPs组和联合暴露组的MDA和PC水平仍然升高。这些发现突出了在应对海洋环境压力源时考虑多种污染物相互作用影响的重要性。
