Cantey J R, Hosterman D S
Infect Immun. 1979 Dec;26(3):1099-103. doi: 10.1128/iai.26.3.1099-1103.1979.
We have reported previously the isolation of an Escherichia coli strain (RDEC-1) that in rabbits is able to colonize the gut, adhere to mucosal epithelial cells of the ileum, cecum, and colon, and cause diarrhea by a novel mechanism. The purpose of the present study was to characterize more fully the colonization of the rabbit gut by strain RDEC-1. Colonization reached a maximum 3 to 4 days post-inoculation with strain RDEC-1 and did not decrease until 15 days post-inoculation. Diarrhea occurred 3 to 4 days after colonization reached its maximum intensity. Semiquantitative rectal swab cultures were found to be correlated with counts of colony-forming units of strain RDEC-1 per gram of ileum, cecum, and colon and their contents and were used to chart the course of colonization of the rabbit gut. The actual number of colony-forming units per gram was dependent on the stage of colonization and ranged from 4.0 X 10(3) to 2.4 X 20(6) in the ileum to 3.1 X 10(5) to 3.6 X 10(7) in the cecum. The number of colony-forming units per gram was not affected by the presence of diarrhea. E. coli RDEC-1 colonizes the ileum, cecum, and colon of rabbits heavily for a relatively long period of time.
我们之前报道过分离出一种大肠杆菌菌株（RDEC-1），该菌株在兔子体内能够定殖于肠道，黏附于回肠、盲肠和结肠的黏膜上皮细胞，并通过一种新机制导致腹泻。本研究的目的是更全面地描述RDEC-1菌株在兔子肠道内的定殖情况。接种RDEC-1菌株后3至4天定殖达到高峰，直到接种后15天才开始下降。腹泻在定殖达到最大强度后3至4天出现。发现半定量直肠拭子培养结果与每克回肠、盲肠和结肠及其内容物中RDEC-1菌株的菌落形成单位计数相关，并用于绘制兔子肠道定殖的进程。每克的实际菌落形成单位数量取决于定殖阶段，在回肠中范围为4.0×10³至2.4×10⁶，在盲肠中为3.1×10⁵至3.6×10⁷。每克的菌落形成单位数量不受腹泻存在的影响。大肠杆菌RDEC-1在兔子的回肠、盲肠和结肠中大量定殖相当长一段时间。
