Tamaki Yuki, Hatayama Naoyuki, Fujii Yutaka, Naito Munekazu
Department of Anatomy, Aichi Medical University, Nagakute, Aichi, 480-1195, Japan.
Department of Clinical Engineering and Medical Technology, Niigata University of Health and Welfare, Niigata, Japan.
J Artif Organs. 2025 Jun;28(2):244-251. doi: 10.1007/s10047-024-01474-8. Epub 2024 Oct 4.
We established a compact machine perfusion system for whole blood perfusion of rat liver by making use of oxygenation filters as an artificial lung. Livers removed from rats were divided into Krebs-Henseleit (control), 50% blood (hemoglobin: 7 g/dL), and whole blood (hemoglobin: 14 g/dL) groups, then perfused (total perfusate volume: 25 ml) with a small oxygenation filter at 37 °C for 120 min. Blood or perfusate was collected over time, and blood gas and blood cell were measured. In addition, bile volume and portal venous pressure measurements were taken. In all groups, the partial pressure of oxygen was controlled to approximately 400 mmHg. Flow rates were maintained at approximately about 20-30 ml/min according to liver size. Portal venous pressure was normal in the 50% blood and whole blood groups, while lower than the reference value in the Krebs-Henseleit group. Twice as much bile was produced in the 50% blood and whole blood groups relative with the Krebs-Henseleit group. We observed no differences in hemoglobin and red blood cell levels. Lactate levels were normal in the 50% blood and whole blood groups, but were elevated in the Krebs-Henseleit group. Our compact perfusion system using oxygenation filters was able to maintain rat liver function by perfusing a small amount of extracorporeal blood. This system is simple and stable, and may contribute to the future development of machine perfusion systems.
我们利用氧合过滤器作为人工肺，建立了一种用于大鼠肝脏全血灌注的紧凑型机器灌注系统。将从大鼠身上取出的肝脏分为克氏-亨氏液（对照组）、50%血液（血红蛋白：7 g/dL）和全血（血红蛋白：14 g/dL）组，然后在37℃下用小型氧合过滤器灌注（总灌注液体积：25 ml）120分钟。随着时间的推移收集血液或灌注液，并测量血气和血细胞。此外，还进行了胆汁量和门静脉压力测量。在所有组中，氧分压控制在约400 mmHg。根据肝脏大小，流速维持在约20-30 ml/min。50%血液组和全血组的门静脉压力正常，而克氏-亨氏液组低于参考值。50%血液组和全血组产生的胆汁量是克氏-亨氏液组的两倍。我们观察到血红蛋白和红细胞水平没有差异。50%血液组和全血组的乳酸水平正常，但克氏-亨氏液组升高。我们使用氧合过滤器的紧凑型灌注系统能够通过灌注少量体外血液来维持大鼠肝脏功能。该系统简单且稳定，可能有助于机器灌注系统的未来发展。
