临床金黄色葡萄球菌分离株产剥脱毒素的筛选：一项方法学研究。

Screening of clinical S. aureus-isolates for the production of exfoliative toxin. A methodological study.

作者信息

Elsner P, Hartmann A A, Lenz W, Brandis H

出版信息

Zentralbl Bakteriol Mikrobiol Hyg A. 1985 Oct;260(2):216-20. doi: 10.1016/s0176-6724(85)80117-6.

Abstract

Screening S. aureus-isolates for the production of exfoliative toxin (ET) and discrimination between its two known variants (ETA, ETB) by immunodiffusion (ID), isoelectric focusing (IEF) and animal experiment were assessed methodologically using isolates from a patient with bullous impetigo and a patient with Ritter von Rittershain's disease. Only by animal assay one of the isolates could be identified as ET-producer. ID was used for the discrimination between ETA and ETB. It is concluded that the in-vivo assay for ET-production using live staphylococci can not yet be replaced by ID and IFE and that in patients with staphylococcal scalded skin syndrome swabs should be taken from multiple lesions und all S. aureus isolates obtained should be screened for ET-production to prevent false-negative results. Lysotyping alone gives no proof of ET-production since types II 55/71 and II 3A/3C do not produce ET in all cases and toxinogenic S. aureus strains of phage groups I and III have been described.

摘要

通过免疫扩散（ID）、等电聚焦（IEF）和动物实验，对金黄色葡萄球菌分离株进行剥脱毒素（ET）产生的筛查，并区分其两种已知变体（ETA、ETB），使用来自大疱性脓疱病患者和 Ritter von Rittershain 病患者的分离株进行了方法学评估。仅通过动物试验，其中一株分离株可被鉴定为 ET 产生菌。ID 用于区分 ETA 和 ETB。得出结论，使用活葡萄球菌进行 ET 产生的体内试验尚不能被 ID 和 IFE 取代，对于葡萄球菌烫伤样皮肤综合征患者，应从多个皮损处采集拭子，并对所有获得的金黄色葡萄球菌分离株进行 ET 产生的筛查，以防止假阴性结果。仅溶菌酶分型不能证明 ET 的产生，因为 II 55/71 型和 II 3A/3C 型并非在所有情况下都产生 ET，并且已经描述了噬菌体组 I 和 III 的产毒素金黄色葡萄球菌菌株。