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超声联合青稞膳食纤维对低盐鸡胸肉肌原纤维蛋白凝胶特性的影响。

Effect of ultrasound combined with highland barley dietary fiber on gel properties of reduced-salt chicken breast myofibrillar protein.

机构信息

College of Tourism and Culinary Science, Yangzhou University, Yangzhou, P. R. China.

Key Laboratory of Chinese Cuisine Intangible Cultural Heritage Technology Inheritance, Ministry of Culture and Tourism, Yangzhou, P. R. China.

出版信息

J Food Sci. 2024 Nov;89(11):7360-7371. doi: 10.1111/1750-3841.17434. Epub 2024 Oct 3.

Abstract

This study aimed to investigate the effect of ultrasound combined with highland barley dietary fiber (HBDF) on the quality of reduced-salt chicken breast myofibrillar protein (MP) gel. The molecular forces maintaining gel structure, the gelling formation process, and gel microstructure of different groups, two control groups (2% sodium chloride [NaCl] group, 1% NaCl group), and four treatment groups (0.3% HBDF+U5, 0.3% HBDF+U10, 0.5% HBDF+U5, and 0.5% HBDF+U10) were examined. Results indicated significant improvements (p < 0.05) in gel properties such as water-holding capacity, textural characteristics, and color of the MP gel of the four treatment groups compared to Control 2 (1% NaCl) group. Furthermore, the second structural alterations were characterized by increase β-sheet, β-turn, and random coil structure contents in treatment groups, especially in 0.3% HBDF+U5 and 0.5% HBDF+U5 groups; in addition, the exposure of more hydrophobic groups and the formation of disulfide bonds and hydrogen bonds were promoted in treatment groups, thus enhancing protein aggregation and gel quality. Finally, compared to Control 2 (1% NaCl) group, more compact and uniform gel network structures and pores inside the composite gels were observed in treatment groups. In conclusion, the findings demonstrated that the application of ultrasound in combination with HBDF improved the gelling characteristics of reduced-salt chicken breast MP gel, especially 0.3% HBDF+U5 and 0.5% HBDF+U5 groups.

摘要

本研究旨在探讨超声联合青稞膳食纤维(HBDF)对低盐鸡胸肉肌原纤维蛋白(MP)凝胶品质的影响。通过对两个对照组(2%氯化钠[NaCl]组和 1%NaCl 组)和四个处理组(0.3%HBDF+U5、0.3%HBDF+U10、0.5%HBDF+U5 和 0.5%HBDF+U10)的分子力维持凝胶结构、胶凝形成过程和凝胶微观结构进行研究。结果表明,与对照组 2(1%NaCl)相比,四个处理组的凝胶性能(持水力、质构特性和 MP 凝胶颜色)均有显著提高(p<0.05)。此外,处理组的二级结构变化特征是β-折叠、β-转角和无规卷曲结构含量增加,特别是在 0.3%HBDF+U5 和 0.5%HBDF+U5 组中;此外,处理组促进了更多疏水性基团的暴露以及二硫键和氢键的形成,从而增强了蛋白质聚集和凝胶质量。最后,与对照组 2(1%NaCl)相比,处理组的复合凝胶内部具有更致密和均匀的凝胶网络结构和孔。综上所述,超声联合 HBDF 的应用改善了低盐鸡胸肉 MP 凝胶的胶凝特性,特别是 0.3%HBDF+U5 和 0.5%HBDF+U5 组。

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