Cereal Crops Research Unit, USDA-ARS, 502 Walnut Street, Madison, WI, 53726, USA.
Department of Genetics, University of Wisconsin-Madison, 425 Henry Mall, WI, Madison, 53726, USA.
BMC Res Notes. 2024 Oct 3;17(1):289. doi: 10.1186/s13104-024-06955-w.
Seeds host microbes that function in plant growth and phytopathogen resistance. The aim of the work was to investigate total bacterial community in malting barley seeds and whether their bacterial seed endophytes have dual functional roles in plant growth-promotion and inhibition of Fusarium graminearum, the causative agent of Fusarium head blight (FHB) in barley. We used culture dependent and culture independent methods.
Phylogenetic classification of seed endophytic bacteria based on sequencing data identified B. subtilis, B. licheniformis and B. pumilis as predominant subgroups. Location driven divergence in bacterial endophytic communities was evident based on a clear separation of the samples from Crookston and other location samples. The bio-primed seeds using one hundred and seventy bacterial isolates showed that 3.5% (6/170) of the bacterial isolates conferred greater than 10% increase in both root length (RL) and shoot length (SL), while 19.4% (33/170) and 26.5% (45/170) showed RL and SL specific growth effects, respectively, relative to controls. Among the six bacterial isolates that increased RL and SL, five (#29, #63, #109, #124 and #126) also significantly inhibit the growth of F. graminearum based on in vitro assays. This study identified novel seed bacterial endophytes that could be further exploited for promoting growth during seedling establishment and as biocontrol for combating the devastating scab disease.
种子中携带有在植物生长和植物病原体抗性中起作用的微生物。本研究旨在调查发芽大麦种子中的总细菌群落，以及它们的细菌种子内生菌是否具有促进植物生长和抑制禾谷镰刀菌（引起大麦赤霉病的病原体）的双重功能。我们使用了依赖培养和非依赖培养的方法。
基于测序数据的种子内生细菌的系统发育分类确定了枯草芽孢杆菌、地衣芽孢杆菌和短小芽孢杆菌是主要的亚群。基于样本在科罗克顿和其他地点样本之间的明显分离，明显存在基于位置的细菌内生群落的趋异。使用 170 个细菌分离株对生物引发的种子进行处理，结果表明，3.5%（6/170）的细菌分离株使根长（RL）和茎长（SL）分别增加了 10%以上，而 19.4%（33/170）和 26.5%（45/170）分别对 RL 和 SL 具有特定的生长效应，与对照相比。在增加 RL 和 SL 的六个细菌分离株中，有五个（#29、#63、#109、#124 和#126）也根据体外测定显著抑制禾谷镰刀菌的生长。本研究鉴定了新的种子细菌内生菌，它们可以进一步用于促进幼苗建立期间的生长，并作为防治毁灭性黑穗病的生物防治剂。
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