Onimisi Onyinoyi Bethel, Musa Sunday Abraham, Umana Uduak Emmanuel, Sambo Sonhap James, Makena Wusa
Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria.
Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University, Zaria, Nigeria.
Anat Cell Biol. 2024 Dec 31;57(4):543-558. doi: 10.5115/acb.24.013. Epub 2024 Oct 4.
Stroke is the most significant cause of disability worldwide. Despite mounting data supporting memory deficit after stroke, dysfunction and treatment effect mechanisms remain unknown. Phenolics can be found in a variety of fruits and vegetables. There is, however, a scarcity of research on the therapeutic potential of the phenolics fraction of (PTT) fruit against ischemic stroke-induced abnormalities in hippocampal tissue. The rats were divided into five groups: Group I, vehicle; group II, ischemia/reperfusion (I/R)+vehicle; group III, I/R+50 mg/kg minocycline (MNC); group IV, I/R+100 mg/kg PTT; and group V, I/R+200 mg/kg PTT. Ischemia was induced via bilateral common carotid artery occlusion for 30 minutes followed by reperfusion. PTT and MNC were intraorally administered daily for 7 days. Neurodegenerative changes, cornu ammonis 1 (CA1) and cornu ammonis 3 (CA3) pyramidal cell count, levels of oxidative stress indicators, and memory functions were assessed. Rats treated with PTT, as well as MNC compared to untreated I/R rats, showed a substantial (<0.05) rise in catalase, superoxide dismutase, glutathione levels, as well as decreased lipid peroxidation and improved memory. I/R resulted in histoarchitectural distortions, a marked decrease (<0.05) in the intensity of the Nissl substance, and a striking decrease (<0.05) in the number of pyramidal cells in the CA1 and CA3. PTT and MNC-treated groups showed significant attenuation in all the above parameters. Taking together, these findings revealed that PTT attenuated oxidative stress, histologic alterations and substantially restored memory impairment in the I/R rat model.
中风是全球致残的最重要原因。尽管越来越多的数据支持中风后存在记忆缺陷，但功能障碍和治疗效果机制仍不清楚。酚类物质存在于多种水果和蔬菜中。然而，关于番荔枝（PTT）果实酚类成分对缺血性中风诱导的海马组织异常的治疗潜力的研究却很匮乏。将大鼠分为五组：第一组，赋形剂组；第二组，缺血/再灌注（I/R）+赋形剂组；第三组，I/R+50毫克/千克米诺环素（MNC）组；第四组，I/R+100毫克/千克PTT组；第五组，I/R+200毫克/千克PTT组。通过双侧颈总动脉闭塞30分钟诱导缺血，随后进行再灌注。PTT和MNC每天经口给药，持续7天。评估神经退行性变化、海马1区（CA1）和海马3区（CA3）锥体细胞计数、氧化应激指标水平以及记忆功能。与未治疗的I/R大鼠相比，接受PTT以及MNC治疗的大鼠过氧化氢酶、超氧化物歧化酶、谷胱甘肽水平显著升高（<0.05），脂质过氧化降低，记忆改善。I/R导致组织结构扭曲，尼氏体强度显著降低（<0.05），CA1和CA3区锥体细胞数量显著减少（<0.05）。PTT和MNC治疗组在上述所有参数方面均显示出显著改善。综上所述，这些发现表明PTT可减轻氧化应激、组织学改变，并显著恢复I/R大鼠模型中的记忆损伤。
