Saladin Lazare, Le Berruyer Valentine, Bonnevial Maxence, Didier Pascal, Collot Mayeul
Chemistry of Photoresponsive Systems, Laboratoire de Chémo-Biologie Synthétique et Thérapeutique (CBST) UMR 7199, CNRS, Université de Strasbourg, F-67400, Illkirch, France.
Laboratoire de Bioimagerie et Pathologies, UMR 7021, CNRS, Université de Strasbourg, 74 route du Rhin, 67401, Illkirch-Graffenstaden, France.
Chemistry. 2024 Dec 18;30(71):e202403409. doi: 10.1002/chem.202403409. Epub 2024 Nov 7.
Photoactivatable fluorescent probes are valuable tools in bioimaging for tracking cells down to single molecules and for single molecule localization microscopy. For the latter application, green emitting dyes are in demand. We herein developed an efficient green-emitting photoactivatable furanyl-BODIPY (PFB) and we established a new mechanism of photoactivation called Directed Photooxidation Induced Activation (DPIA) where the furan is photo-oxidized in a directed manner by the singlet oxygen produced by the probe. The efficient photoconverter (93-fold fluorescence enhancement at 510 nm, 49 % yield conversion) is functionalizable and allowed targeting of several subcellular structures and organelles, which were photoactivated in live cells. Finally, we demonstrated the potential of PFB in super-resolution imaging by performing PhotoActivated Localization Microscopy (PALM) in live cells.
光可激活荧光探针是生物成像中用于追踪细胞直至单分子以及用于单分子定位显微镜的宝贵工具。对于后一种应用，需要发射绿色荧光的染料。我们在此开发了一种高效发射绿色荧光的光可激活呋喃基硼二吡咯（PFB），并建立了一种新的光激活机制，称为定向光氧化诱导激活（DPIA），其中呋喃被探针产生的单线态氧以定向方式光氧化。这种高效的光转换器（在510 nm处荧光增强93倍，产率转换为49%）具有功能化能力，可靶向几种亚细胞结构和细胞器，这些结构和细胞器在活细胞中被光激活。最后，我们通过在活细胞中进行光激活定位显微镜（PALM）证明了PFB在超分辨率成像中的潜力。
