Wu Deyan, He Xuemin, Jiang Lamei, Li Wenjing, Wang Hengfang, Lv Guanghui
College of Ecology and Environment, Xinjiang University, Urumqi, Xinjiang, China.
Key Laboratory of Oasis Ecology of Ministry of Education, Xinjiang University, Urumqi, Xinjiang, China.
Front Plant Sci. 2024 Sep 19;15:1461893. doi: 10.3389/fpls.2024.1461893. eCollection 2024.
Root exudates act as the "language" of plant-soil communication, facilitating crucial interactions, information exchange, and energy transfer between plants and soil. The interactions facilitated by root exudates between plants and microorganisms in the rhizosphere are crucial for nutrient uptake and stress resilience in plants. However, the mechanism underlying the interaction between root exudates and rhizosphere microorganisms in desert plants under drought conditions remains unclear, especially among closely related species.
To reveal the ecological strategies employed by the genus Haloxylon in different habitats. Using DNA extraction and sequencing and UPLC-Q-Tof/MS methods, we studied root exudates and soil microorganisms from two closely related species, and , to assess differences in their root exudates, soil microbial composition, and interactions.
Significant differences were found in soil properties and root traits between the two species, among which soil water content (SWC) and soil organic carbon (SOC) in rhizosphere and bulk soils ( < 0.05). While the metabolite classification of root exudates was similar, their components varied, with terpenoids being the main differential metabolites. Soil microbial structure and diversity also exhibited significant differences, with distinct key species in the network and differential functional processes mainly related to nitrogen and carbon cycles. Strong correlations were observed between root exudate-mediated root traits, soil microorganisms, and soil properties, although the complex interactions differed between the two closely relative species. The primary metabolites found in the network of include sugars and fatty acids, while relies on secondary metabolites, steroids and terpenoids.
These findings suggest that root exudates are key in shaping rhizosphere microbial communities, increasing microbial functionality, fostering symbiotic relationships with hosts, and bolstering the resilience of plants to environmental stress.
根系分泌物充当植物与土壤交流的“语言”，促进植物与土壤之间关键的相互作用、信息交换和能量传递。根系分泌物促进的植物与根际微生物之间的相互作用，对于植物养分吸收和胁迫恢复力至关重要。然而，干旱条件下荒漠植物根系分泌物与根际微生物之间相互作用的潜在机制仍不清楚，尤其是在亲缘关系较近的物种之间。
为揭示梭梭属植物在不同生境中采用的生态策略。利用DNA提取与测序以及超高效液相色谱-四极杆飞行时间串联质谱（UPLC-Q-Tof/MS）方法，我们研究了两个亲缘关系较近的物种梭梭（Haloxylon ammodendron）和白梭梭（Haloxylon persicum）的根系分泌物和土壤微生物，以评估它们根系分泌物、土壤微生物组成及相互作用的差异。
在两个物种之间的土壤性质和根系性状方面发现了显著差异，其中根际土壤和原状土壤中的土壤含水量（SWC）和土壤有机碳（SOC）（P<0.05）。虽然根系分泌物的代谢物分类相似，但其成分有所不同，萜类化合物是主要的差异代谢物。土壤微生物结构和多样性也表现出显著差异，网络中有不同的关键物种，且差异功能过程主要与氮和碳循环有关。尽管两个亲缘关系较近的物种之间的复杂相互作用有所不同，但在根系分泌物介导的根系性状、土壤微生物和土壤性质之间观察到了强烈的相关性。梭梭网络中发现的主要代谢物包括糖类和脂肪酸，而白梭梭则依赖于次生代谢物、类固醇和萜类化合物。
这些发现表明，根系分泌物对于塑造根际微生物群落、增加微生物功能、促进与宿主的共生关系以及增强植物对环境胁迫的恢复力至关重要。
