He Dongmei, Gao Weiping, Zhang Zhanling, Xing Jinniu, Han Guiqi, Wang Hai, Yan Zhuyun
State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.
School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.
PeerJ. 2025 Mar 31;13:e19208. doi: 10.7717/peerj.19208. eCollection 2025.
Transplanting is important for obtaining and maintaining excellent germplasm of cultivated plants. During plant transplantation, the endophytic microbial community regularly reorganizes, which may be crucial for plant germplasm rejuvenation. , a widely used medicinal and edible plant, relies on transplanting for its exceptional quality. To explore the microecological recombination of during the transplanting process of "alpine seedling-cellar planting-dam cultivation", this study analyzed shifts in endophytic and soil microbial communities across the three transplanting stages in Min County, Gansu Province, China. High-throughput sequencing revealed significant changes, with 82.27% to 84.65% of bacteria and 93.19% to 93.49% of fungi species altering in transplanted . Main findings indicate that Mortierellomycota, Actinobacteriota, and Myxococcota were dominant in cellar planting root and cellar rhizosphere soil, contrasting with Firmicutes predominance in alpine and dam areas. Notably, potentially pathogenic endophytes like and decreased post-alpine seedling and cellar planting, favoring a healthier plant environment. Cellar planting root exhibited a rich accumulation of psychrophilic flora, including , , and , alongside a unique dominance of fungi. Microbial co-occurrence network analysis highlighted cellar planting root as pivotal, suggesting its importance in microbial interactions. In conclusion, transplanting significantly reshaped 's endophytic flora, with fungi showing more pronounced recombination than bacteria. Soil microbial communities emerged as crucial drivers of this recombination, facilitating the overwintering of , reducing diseases, and rejuvenating the germplasm. Transplanting-driven microecological reorganization is an important scientific mechanism for the high-quality production of cultivated medicinal plants.
移植对于获取和保持栽培植物的优良种质很重要。在植物移植过程中，内生微生物群落会定期重组，这可能对植物种质复壮至关重要。 是一种广泛使用的药食两用植物，其卓越品质依赖于移植。为了探究“高山育苗-地窖种植-坝地栽培”移植过程中 的微生态重组情况，本研究分析了中国甘肃省岷县三个移植阶段内生和土壤微生物群落的变化。高通量测序显示出显著变化，移植后的 中有82.27%至84.65%的细菌种类和93.19%至93.49%的真菌种类发生了改变。主要研究结果表明，被孢霉门、放线菌门和黏球菌门在地窖种植根和地窖根际土壤中占主导地位，这与高山和坝地区域以厚壁菌门为主形成对比。值得注意的是，像 和 等潜在致病内生菌在高山育苗和地窖种植后减少，有利于营造更健康的植物环境。地窖种植根表现出嗜冷菌群的丰富积累，包括 、 和 ，同时还有 真菌的独特优势。微生物共现网络分析突出了地窖种植根的关键作用，表明其在微生物相互作用中的重要性。总之，移植显著重塑了 的内生菌群，真菌的重组比细菌更为明显。土壤微生物群落成为这种重组的关键驱动因素，促进了 的越冬、减少了病害并使种质复壮。移植驱动的微生态重组是栽培药用植物高质量生产的重要科学机制。
