Chang Xiling, Zhang Shuangxing, Cao Changyu, Zhou Jianfei, Wang Xiaoxing, Zhang Dingguo, Xiang Jishan
College of Biological Sciences and Technology, Yili Normal University, Yili, 830500, Xinjiang, China.
College of Agronomy, Northwest A & F University, Yangling, 712100, Shanxi, China.
Heliyon. 2024 Sep 19;10(18):e38083. doi: 10.1016/j.heliyon.2024.e38083. eCollection 2024 Sep 30.
Foxtail millet [] plays a crucial role as a multigrain crop in agricultural production. However, due to future extreme weather conditions, drought remains the main abiotic stress that limits foxtail millet yield, it is highly significant to screen for drought-tolerant varieties throughout the entire growth period and identify the regulatory genes associated with drought resistance in foxtail millet breeding. We identified 217 foxtail millet seed resources for drought resistance during the maturity stage in the field, and subsequently categorized them into different levels of drought resistance. Two cultivars with extremely strong drought resistance during the maturity stage in the field, JKH4 (Chi 5422) and JKH6 (Chigu 26), as well as two cultivars with extremely weak drought resistance during the maturity stage in the field, JRK3 (17M1309) and JRK6 (Canggu 9), were selected for physiological comparison and transcriptome sequencing before and after drought treatment. Transcriptome analysis at the seedling stage revealed that JRK3 and JRK6 cultivar primarily regulated phenylpropanoid biosynthesis, MAPK signaling pathogen-plant, and plant hormone signal transduction pathway in response to drought stress. On the other hand, the fatty acid elongation pathway of JKH4 and JKH6 variety was found to be more significant. Furthermore, 22 drought resistance related genes were screened through transcriptome analysis of four foxtail millet varieties. These findings could offer valuable theoretical guidance for breeding foxtail millet with enhanced drought resistance and potentially facilitate the development of genetically engineered drought-resistant foxtail millet varieties.
谷子作为一种多谷物作物，在农业生产中发挥着关键作用。然而，由于未来极端天气条件的影响，干旱仍然是限制谷子产量的主要非生物胁迫，在整个生育期筛选耐旱品种并鉴定谷子育种中与抗旱相关的调控基因具有重要意义。我们在田间成熟期鉴定了217份谷子种子资源的抗旱性，随后将它们分为不同的抗旱水平。选择了两个在田间成熟期具有极强抗旱性的品种JKH4（赤5422）和JKH6（赤谷26），以及两个在田间成熟期具有极弱抗旱性的品种JRK3（17M1309）和JRK6（沧谷9），进行干旱处理前后的生理比较和转录组测序。苗期转录组分析表明，JRK3和JRK6品种在响应干旱胁迫时主要调控苯丙烷生物合成、MAPK信号通路-植物病原体互作和植物激素信号转导途径。另一方面，发现JKH4和JKH6品种的脂肪酸延长途径更为显著。此外，通过对四个谷子品种的转录组分析筛选出22个与抗旱相关的基因。这些发现可为培育抗旱性增强的谷子提供有价值的理论指导，并可能促进转基因抗旱谷子品种的开发。
