Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Food for State Market Regulation, Wuhan, 430070, China.
Wuhan Academy of Agricultural Sciences, Wuhan, 430072, China.
Anal Methods. 2024 Nov 14;16(44):7502-7508. doi: 10.1039/d4ay01334d.
Cr(VI) is one of the most typical heavy metal contaminants and rapid detection of Cr(VI) is highly important in food control and public health. Herein, a core-shell Au@Pt nanozyme-based colorimetric assay was developed for the rapid and sensitive detection of Cr(VI). The monodispersed Au@Pt core-shell nanoparticles exhibited high peroxidase-mimicking activity and can catalyze colorless TMB into blue-colored oxidized oxTMB. After the addition of Cr(VI), the oxTMB molecules can be reduced into colorless TMB. The ultrathin Pt shell can prevent the Pt component from aggregation, thus improving the catalytic activity of Au@Pt nanozyme. These Au@Pt nanozyme-based Cr(VI) assays exhibited high sensitivity and selectivity and displayed satisfactory recoveries in practical samples. Our work highlights opportunities for the development of core-shell nanozymes with extensive applications in food safety, biomedicine, and environmental monitoring.
六价铬（Cr(VI)）是最典型的重金属污染物之一，快速检测 Cr(VI)对于食品控制和公共卫生非常重要。在此，开发了一种基于核壳 Au@Pt 纳米酶的比色测定法，用于快速灵敏地检测 Cr(VI)。单分散的 Au@Pt 核壳纳米粒子表现出高过氧化物酶模拟活性，并能催化无色 TMB 生成蓝色氧化 oxTMB。加入 Cr(VI)后，oxTMB 分子可被还原成无色 TMB。超薄的 Pt 壳可以防止 Pt 组分聚集，从而提高 Au@Pt 纳米酶的催化活性。基于这些 Au@Pt 纳米酶的 Cr(VI)测定法具有高灵敏度和选择性，并在实际样品中显示出令人满意的回收率。我们的工作突出了核壳纳米酶在食品安全、生物医药和环境监测等广泛应用中的发展机遇。
