Department of Oral Medicine and Periodontology, Faculty of Dentistry, Sabratha University, Sabratha, Libya, Orcid: https://orcid.org/0009-0007-9083-1973.
Department of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt; Department of Oral Biology, Faculty of Oral and Dental Medicine, Delta University for Science and Technology, Mansoura, Egypt, Orcid: https://orcid.org/0000-0003-4732-8022.
J Contemp Dent Pract. 2024 May 1;25(5):424-431. doi: 10.5005/jp-journals-10024-3697.
This study compared the quality and quantity of newly formed bone in rabbits' critical-sized calvarial defects filled with enamel matrix derivative (EMD) combined with freeze-dried bone allograft (FDBA) vs FDBA alone.
A total of 24 adult male white New Zealand rabbits were included. In each rabbit, three bone defects with a diameter of 8 mm were created on the calvarium bone; the first defect was left untreated, while the second was filled with FDBA, and the third was filled with EMD + FDBA. Twelve rabbits were randomly euthanized after a month, and the remaining 2 month postsurgery. Bone sections were histologically evaluated by hematoxylin and eosin and vascular endothelial growth factor (VEGF), alkaline phosphatase (ALP), osteoprotegerin (OPG), and receptor activator of NF-kappaB (RANK) immune-histochemical staining.
An improvement in the newly formed bone percentage was found in the defects filled with EMD + FDBA in comparison with FDBA and control defects at 1 month and 2 months postsurgery. Additionally, the expression of VEGF, ALP, OPG, and RANK showed highly significant differences in the defects filled with EMD + FDBA compared to the FDBA and control ones at 1 month postsurgery ( = 0.001). Meanwhile, VEGF and ALP expression showed a significant decrease in defects filled with EMD + FDBA compared to the FDBA and control ones ( = 0.001), while OPG and RANK expression showed non-significant differences between treated groups at 2 months postsurgery.
Enamel matrix derivative combined with FDBA has a synergistic effect on bone formation and graft substitution. This combination accelerates the expression of VEGF, ALP, OPG, and RANK.
The combination of EMD and FDBA accelerates and ameliorates the quality of newly formed bone, aiding in maxillofacial reconstruction. How to cite this article: Zakri RN, Grawish ME, Mowafey B, et al. Impact of Freeze-dried Corticocancellous Bone Allograft Combined with Enamel Matrix Derivative in the Treatment of Critical-sized Calvarial Bone Defects: An Animal Study. J Contemp Dent Pract 2024;25(5):424-431.
本研究比较了釉基质衍生物（EMD）联合冻干骨移植物（FDBA）与 FDBA 单独填充兔颅骨临界尺寸骨缺损时新形成骨的质量和数量。
共纳入 24 只成年雄性新西兰白兔。在每只兔子的颅骨上共创建 3 个直径为 8mm 的骨缺损；第一个缺损不做处理，第二个缺损填充 FDBA，第三个缺损填充 EMD+FDBA。术后 1 个月和 2 个月时，随机处死 12 只兔子，其余 2 只继续观察。通过苏木精-伊红染色和血管内皮生长因子（VEGF）、碱性磷酸酶（ALP）、护骨素（OPG）和核因子-κB 受体激活剂（RANK）免疫组织化学染色对骨切片进行组织学评估。
术后 1 个月和 2 个月，与 FDBA 和对照组相比，EMD+FDBA 填充的缺损中新生骨百分比有改善。此外，术后 1 个月，与 FDBA 和对照组相比，EMD+FDBA 填充的缺损中 VEGF、ALP、OPG 和 RANK 的表达差异具有统计学意义（=0.001）。同时，与 FDBA 和对照组相比，EMD+FDBA 填充的缺损中 VEGF 和 ALP 的表达显著降低（=0.001），而 OPG 和 RANK 的表达在术后 2 个月时两组之间无显著差异。
釉基质衍生物联合 FDBA 对骨形成和移植物替代具有协同作用。这种组合加速了 VEGF、ALP、OPG 和 RANK 的表达。
EMD 和 FDBA 的联合使用加速并改善了新形成骨的质量，有助于颌面重建。
Zakri RN, Grawish ME, Mowafey B, et al. Impact of Freeze-dried Corticocancellous Bone Allograft Combined with Enamel Matrix Derivative in the Treatment of Critical-sized Calvarial Bone Defects: An Animal Study. J Contemp Dent Pract 2024;25(5):424-431.
