Departmento de Odontología Integral, Universidad de la Frontera, Temuco, Chile.
Ann Anat. 2011 Oct 20;193(5):412-7. doi: 10.1016/j.aanat.2011.06.003. Epub 2011 Jul 8.
The histological behavior of bone formation using three biomaterials was examined including whether an 8mm rabbit calvarial defect would behave as a critical size defect. Four trephine defects of 8mm diameter in were created six rabbit parietal bones. A control defect was maintained only with coagulum and others were filled with autologous bone, FDBA (freeze dried bone allograft) and a mixture of autologous bone with FDBA, respectively. The animals were sacrificed after between 15 and 90 days at intervals of two weeks and the extracted samples were processed for histological evaluation. All control defects showed incomplete bone formation during 90 days of observation. The defects filled with FDBA and mixture of FDBA-autologous bone exhibited a higher regeneration degree than autologous bone after 60 days; however, the only biomaterial revealing complete mineralization of the original defect at 90 days was FDBA. In conclusion, 8mm defects can be considered as critical size defects and only FDBA showed mature lamellar bone at 90 days.
使用三种生物材料检查骨形成的组织学行为，包括 8mm 兔颅骨缺损是否表现为临界尺寸缺损。在 6 块兔顶骨上制作了 4 个 8mm 直径的环钻缺损。一个对照缺损仅用凝血块维持，其他缺损分别用自体骨、FDBA（冻干骨同种异体移植物）和自体骨与 FDBA 的混合物填充。动物在 15 至 90 天之间每隔两周处死一次，提取的样本进行组织学评估。所有对照缺损在 90 天的观察期间均显示不完全骨形成。在 60 天后，填充 FDBA 和 FDBA-自体骨混合物的缺损显示出比自体骨更高的再生程度；然而，在 90 天时唯一显示原始缺损完全矿化的生物材料是 FDBA。总之，8mm 缺损可被视为临界尺寸缺损，只有 FDBA 在 90 天时显示成熟的板层骨。
