Division of Pulmonary, Allergy, and Critical Care Medicine, Department of Medicine, Duke University Medical Center, Durham, NC, USA.
Methods Mol Biol. 2022;2506:67-81. doi: 10.1007/978-1-0716-2364-0_5.
Ozone (O), a criterion air pollutant produced as a product of internal combustion, generates increased inflammation, lung permeability, and airway hyperreactivity when exposed to rodents in laboratory settings. Airway hyperreactivity is defined as an exaggerated acute obstructive response of the airways to one or more nonspecific stimuli. Lung permeability is a measure of barrier functions that separate internal and external environments to limit access of pathogens and other noxious material. By modeling in vivo O exposure in rodents, this allows investigators to explore pulmonary and nonpulmonary O effects as a means of understanding its impact on human health and lung function. Furthermore, direct effects of O on epithelial permeability can be defined using in vitro exposures to airway epithelial cells. This chapter will focus on methods of generating O and then exposing rodents and cultured epithelial cells in laboratory settings.
臭氧（O）是一种作为内燃机产物的空气污染物，当暴露于实验室环境中的啮齿动物时，会引起炎症、肺通透性和气道高反应性增加。气道高反应性定义为气道对一种或多种非特异性刺激物的急性阻塞性反应过度。肺通透性是一种衡量分隔内外环境的屏障功能的指标，以限制病原体和其他有害物质的进入。通过在啮齿动物中模拟体内臭氧暴露，可以让研究人员探索肺部和非肺部臭氧的影响，以此了解其对人类健康和肺功能的影响。此外，还可以使用气道上皮细胞的体外暴露来定义臭氧对上皮通透性的直接影响。本章将重点介绍生成臭氧的方法，然后在实验室环境中暴露于啮齿动物和培养的上皮细胞。
