Newcastle University Biosciences Institute, Newcastle University, Newcastle Upon Tyne, NE2 4AH, UK.
The Medical Research Council Biomedical NMR Centre, The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK.
Biomol NMR Assign. 2024 Dec;18(2):315-321. doi: 10.1007/s12104-024-10206-1. Epub 2024 Oct 4.
Chromosomal replication is a ubiquitous and essential cellular process. In bacteria, the master replication initiator DnaA plays a key role in promoting an open complex at the origin (oriC) and recruiting helicase in a tightly regulated process. The C-terminal domain IV specifically recognises consensus sequences of double-stranded DNA in oriC, termed DnaA-boxes, thereby facilitating the initial engagement of DnaA to oriC. Here, we report the Cβ and backbone H, N, and C chemical shift assignments of soluble DnaA domain IV from Bacillus subtilis at pH 7.6 and 298 K.
染色体复制是一种普遍存在且至关重要的细胞过程。在细菌中，主复制起始因子 DnaA 在促进复制起点 (oriC) 处的开放复合物形成以及在严格调控的过程中招募解旋酶方面发挥着关键作用。C 端结构域 IV 特异性识别 oriC 中双链 DNA 的共识序列，称为 DnaA-box，从而促进 DnaA 与 oriC 的初始结合。在这里，我们报道了 pH 7.6 和 298 K 下来自枯草芽孢杆菌的可溶性 DnaA 结构域 IV 的 Cβ 和主链 H、N 和 C 的化学位移赋值。
