School of Archaeology and Ancient History, University of Leicester, Leicester LE1 7RH, UK.
Leicester Cancer Research Centre, RKCSB, University of Leicester, Leicester LE1 7RH, UK.
Sci Adv. 2024 Oct 4;10(40):eadn9317. doi: 10.1126/sciadv.adn9317.
Tobacco consumption affects human health, but no studies have investigated its effect on the bone metabolome, or if any changes are traceable after long postmortem intervals. Human osteoarchaeological remains preserve small molecules, making them valuable for studies that aim to examine past conditions. We test if there are molecular differences in the metabolome of cortical bone between archaeological individuals who used tobacco and those who did not, and if these differences are distinct enough to assign tobacco use status to individuals with unknown tobacco use. Cortical bone of 323 known and unknown tobacco users was studied by an untargeted metabolomics assay using a liquid chromatography-high-resolution mass spectrometry platform. We identified 45 discriminating molecular features that differed between tobacco consumers (15 up-regulated features) and nonconsumers (17 up-regulated features). Tobacco consumption leaves a metabolic record in human bone distinctive enough to identify its use in individuals of unknown tobacco consumption. Future work will validate molecular features relating to tobacco consumption.
吸烟会影响人体健康，但目前尚无研究调查其对骨骼代谢组的影响，也不知道在长时间的死后间隔后是否会发生任何变化。人类骨骼考古遗存保存着小分子，这使它们成为过去条件研究的宝贵资源。我们检验了在使用和不使用烟草的考古个体的皮质骨代谢组中是否存在分子差异，如果这些差异足够明显，是否可以将烟草使用状态分配给未知烟草使用的个体。我们使用基于液相色谱-高分辨率质谱平台的非靶向代谢组学分析方法，研究了 323 名已知和未知的烟草使用者的皮质骨。我们鉴定出了 45 个区分性分子特征，这些特征在烟草使用者（15 个上调特征）和非使用者（17 个上调特征）之间存在差异。吸烟在人类骨骼中留下了一种代谢记录，足以识别其在未知烟草使用者中的使用情况。未来的工作将验证与烟草使用相关的分子特征。
