Gao Jie, Sun Xiang, Ma Yanning, Qin Wen, Li Jin, Jin Zuolin, Qiu Jun, Zhang Hao
State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, 710032, China.
Department of Stomatology, The First Hospital of Yulin, Yulin, 719000, China.
Mater Today Bio. 2024 Aug 9;28:101195. doi: 10.1016/j.mtbio.2024.101195. eCollection 2024 Oct.
The physical microenvironment, including substrate rigidity and topology, impacts myoblast differentiation and myotube maturation. However, the interplay effect and physical mechanism of mechanical stimuli on myotube formation is poorly understood. In this study, we utilized elastic substrates, microcontact patterning technique, and particle image velocimetry to investigate the effect of substrate rigidity and topological constraints on myoblast behaviors. Our findings suggested the interplay of substrate stiffness and cellular confinement improved the myotube formation by inducing centripetal cellular motility. These results shed light on the impact of the topological substrate on myoblast differentiation and emphasize the critical role of asymmetrical cell motility during this process, which is highly correlated with cell movement and crowding. Our research provides insights into the intricate interplay between substrate properties, cell motility, and myotube formation during myogenesis. Understanding these mechanisms could trigger tissue engineering strategies and therapies to enhance muscle regeneration and function.
包括底物刚度和拓扑结构在内的物理微环境会影响成肌细胞的分化和肌管的成熟。然而，机械刺激对肌管形成的相互作用效应和物理机制仍知之甚少。在本研究中，我们利用弹性底物、微接触印刷技术和粒子图像测速技术，研究底物刚度和拓扑约束对成肌细胞行为的影响。我们的研究结果表明，底物刚度和细胞限制的相互作用通过诱导向心细胞运动促进了肌管的形成。这些结果揭示了拓扑底物对成肌细胞分化的影响，并强调了不对称细胞运动在此过程中的关键作用，这与细胞移动和拥挤高度相关。我们的研究为肌生成过程中底物特性、细胞运动和肌管形成之间的复杂相互作用提供了见解。了解这些机制可能会引发组织工程策略和疗法，以促进肌肉再生和功能恢复。
