Akintonwa D A
Ecotoxicol Environ Saf. 1985 Oct;10(2):145-9. doi: 10.1016/0147-6513(85)90060-0.
It was possible, by reverse-phase high-pressure liquid chromatography (HPLC) to separate thiourea, thioacetamide, and phenobarbitone with retention times of 1.4, 1.7, and 4.7 min, respectively. Thiourea and thioacetamide are known carcinogens. Substances such as phenobarbitone, thiourea, and thioacetamide, which may be encountered in our environment, can now be identified by this technique. It is also recommended that this HPLC technique should be used routinely in the screening of the purity of substances which are being subjected to toxicity, carcinogenicity, mutagenicity, and teratogenicity evaluation. A mixture of impurities in the sodium phenobarbitone was removed in the synthesis of the 5-ethyl-5-phenyl-barbituric acid (phenobarbitone). These substances which were not thiourea or thioacetamide could have been responsible for the positive result in the in vitro degranulation test for carcinogens with sodium phenobarbitone.
通过反相高效液相色谱法（HPLC）能够分离硫脲、硫代乙酰胺和苯巴比妥，其保留时间分别为1.4分钟、1.7分钟和4.7分钟。硫脲和硫代乙酰胺是已知的致癌物。我们环境中可能会遇到的诸如苯巴比妥、硫脲和硫代乙酰胺等物质，现在可以通过这项技术进行鉴定。还建议在对正在进行毒性、致癌性、致突变性和致畸性评估的物质纯度进行筛选时，常规使用这种HPLC技术。在5-乙基-5-苯基巴比妥酸（苯巴比妥）的合成过程中，除去了苯巴比妥钠中的杂质混合物。这些非硫脲或硫代乙酰胺的物质可能是导致苯巴比妥钠致癌物体外脱颗粒试验呈阳性结果的原因。
