Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, Centre of Advanced Study, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, West Bengal, 700019, India.
Food Technology & Science Institute (FTSI), TCG Centres for Research & Education in Science and Technology, ARDC building, 54/A/1, Block-DN, Sector-5, Salt Lake, Kolkata, West Bengal, 700091, India.
Antonie Van Leeuwenhoek. 2024 Oct 5;118(1):15. doi: 10.1007/s10482-024-02018-z.
During extensive field explorations of the Lateritic area in West Bengal, one remarkable wild Russuloid macrofungus, ethnically termed "Kend Patra," was collected. The species was known to enrich the diet of the local people, being considered as income source for some tribal groups. Using morphological characters and molecular analysis of this collection, provide a unique placement of the taxon in the Russula subgenus Compactae (Fr.) Bon. Further in order to find functional constituents for biopharma applications, methanolic extract was prepared that shows the existence of a substantial amounts of phenol, flavonoid, ascorbic acid and carotenoids. Antioxidant activity was determined where the fraction demonstrated strong DPPH, ABTS, and nitric oxide radical scavenging activities, high Fe ion chelating ability, and a reducing power with EC values ranging from 538.69 to 891.75 µg/ml. The extract was found to be effective against Listeria monocytogenes, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and Staphylococcus aureus. In addition, the extract exhibited potent anticancer activities as it inhibited A549 cell proliferation, caused morphological changes, elevated ROS levels, hindered the clonogenic ability and migratory potential of cancerous cells, arrested cell cycle progression at S phase, and induced apoptosis by modulating the intrinsic mitochondrial pathway. Overall, this study contributes a new species to the world's myco-diversity and presents an exciting opportunity for future researchers to conduct comprehensive investigations on this unique species in order to uncover potential new medications for human use.
在西孟加拉邦的红土区进行广泛的野外考察时，采集到了一种引人注目的野生 Russuloid 大型真菌，当地人将其俗称为“Kend Patra”。这种真菌是当地人饮食的重要组成部分，被一些部落群体视为收入来源。通过对该采集物进行形态学特征和分子分析，将该分类单元在 Russula 亚属 Compactae (Fr.) Bon 中进行了独特的定位。此外，为了寻找用于生物制药应用的功能成分，制备了甲醇提取物，该提取物显示出存在大量的酚类、类黄酮、抗坏血酸和类胡萝卜素。抗氧化活性测定表明，该提取物具有强烈的 DPPH、ABTS 和一氧化氮自由基清除活性、高 Fe 离子螯合能力和还原能力，EC 值范围为 538.69 至 891.75 µg/ml。该提取物对李斯特菌、枯草芽孢杆菌、铜绿假单胞菌、大肠杆菌、伤寒沙门氏菌和金黄色葡萄球菌均具有抑制作用。此外，该提取物表现出有效的抗癌活性，因为它抑制了 A549 细胞的增殖，引起形态变化，增加了 ROS 水平，阻碍了癌细胞的集落形成能力和迁移潜力，阻滞了细胞周期在 S 期的进展，并通过调节内在的线粒体途径诱导细胞凋亡。总的来说，这项研究为世界真菌多样性增添了一个新物种，并为未来的研究人员提供了一个令人兴奋的机会，以对这种独特的物种进行全面研究，为人类用途发现潜在的新药物。
