Lin Galvin Sim Siang, Lestari Widya, Muhamad Halil Mohd Haikal, Abd Aziz Mohd Syafiq
Department of Restorative Dentistry, Kulliyyah of Dentistry, International Islamic University Malaysia, Kuantan Campus, 25200, Kuantan, Pahang, Malaysia.
Department of Fundamental Dental and Medical Sciences, Kulliyyah of Dentistry, International Islamic University Malaysia, Kuantan Campus, 25200, Kuantan, Pahang, Malaysia.
Odontology. 2025 Apr;113(2):457-465. doi: 10.1007/s10266-024-01012-1. Epub 2024 Oct 5.
Silver nanoparticles (AgNPs) have gained prominence in endodontics due to their exceptional antimicrobial properties. However, concerns regarding their genotoxic potential have prompted investigations into their safety profiles. This scoping review aims to consolidate current knowledge on the genotoxic effects of AgNPs in the field of endodontics. A thorough literature search across seven electronic databases was conducted using specific keywords. Inclusion criteria included experimental studies published in English from January 1960 to March 2024, addressing the genotoxicity of AgNPs in endodontic applications. Study selection and data extraction were conducted independently. The Quality Assessment Tool For In Vitro Studies (QUIN) tool was employed to evaluate the risk of bias in each study. 5 articles were selected, of which 3 were in vitro experimental designs, while the remaining were ex vivo studies. All were published between 2009 and 2021. AgNPs have been used as root canal irrigating solutions, pulp-capping materials, and root canal sealers. Most studies employed the comet assay for genotoxic evaluation. One study was found to have a low risk of bias, while others were categorized as having a medium risk. Mixed findings were noted on the genotoxic effects of AgNPs using various assays. The genotoxic potential of AgNPs somewhat poses concerns for endodontic practices. This review highlights the need for further research to develop safer alternatives and optimize their concentrations and exposure durations.
由于其卓越的抗菌性能，银纳米颗粒（AgNPs）在牙髓病学领域备受关注。然而，对其遗传毒性潜力的担忧促使人们对其安全性进行调查。本范围综述旨在整合牙髓病学领域中关于AgNPs遗传毒性作用的现有知识。使用特定关键词对七个电子数据库进行了全面的文献检索。纳入标准包括1960年1月至2024年3月以英文发表的实验研究，探讨AgNPs在牙髓病应用中的遗传毒性。研究选择和数据提取独立进行。采用体外研究质量评估工具（QUIN）评估每项研究的偏倚风险。共筛选出5篇文章，其中3篇为体外实验设计，其余为离体研究。所有文章均发表于2009年至2021年之间。AgNPs已被用作根管冲洗液、盖髓材料和根管封闭剂。大多数研究采用彗星试验进行遗传毒性评估。发现一项研究的偏倚风险较低，而其他研究则被归类为具有中等风险。使用各种检测方法对AgNPs的遗传毒性作用得出了混合结果。AgNPs的遗传毒性潜力在一定程度上给牙髓病治疗带来了担忧。本综述强调需要进一步研究以开发更安全的替代品，并优化其浓度和暴露时间。
