Alkan Hatice, Ciğerci İbrahim Hakkı, Ali Muhammad Muddassir, Hazman Omer, Liman Recep, Colă Florica, Bonciu Elena
Faculty of Science and Literature, Molecular Biology and Genetics Department, Afyon Kocatepe University, Afyonkarahisar 03200, Turkey.
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences Lahore, Punjab 54000, Pakistan.
Plants (Basel). 2022 May 12;11(10):1293. doi: 10.3390/plants11101293.
Nowadays, green synthesized nanoparticles (NPs) are extensively investigated to explore their biological potential. They are being explored to treat different infectious and cancerous diseases. Therefore, the current study was designed to evaluate the cytotoxic and genotoxic effects of biosynthesized silver nanoparticles (AgNPs) from the medicinal plant on breast cancer (MCF-7) and HUVEC (human umbilical vein endothelial cells) cell lines. -mediated AgNPs were synthesized from the extract (MOE) and then characterized through the use of a scanning electron microscope (SEM), X-ray diffraction (XRD) and UV-vis spectrophotometer. Biosynthesized AgNPs and MOE were employed on MCF-7 and HUVEC cell lines to evaluate their cytotoxic and genotoxic effects. More cytotoxic effects were observed by AgNPs and MOE on MCF-7 cell lines. The IC for biosynthesized AgNPs was found to be 5 μg/mL. DNA damage was also observed by the MOE and AgNPs on MCF-7 cell lines. However, non-significant DNA damage was observed by MOE and AgNPs on HUVEC cell lines. The findings of the current study revealed the cytotoxic and genotoxic effects of biosynthesized AgNPs on MCF-7 cell lines. However, these AgNPs were considered safe for normal HUVEC cell lines.
如今，人们广泛研究绿色合成纳米颗粒（NPs）以探索其生物学潜力。正在探索用它们来治疗不同的传染病和癌症。因此，本研究旨在评估药用植物生物合成的银纳米颗粒（AgNPs）对乳腺癌（MCF-7）和人脐静脉内皮细胞（HUVEC）细胞系的细胞毒性和遗传毒性作用。通过[植物名称]提取物（MOE）介导合成AgNPs，然后使用扫描电子显微镜（SEM）、X射线衍射（XRD）和紫外可见分光光度计对其进行表征。将生物合成的AgNPs和MOE应用于MCF-7和HUVEC细胞系，以评估它们的细胞毒性和遗传毒性作用。观察到AgNPs和MOE对MCF-7细胞系有更强的细胞毒性作用。发现生物合成的AgNPs的半数抑制浓度（IC）为5μg/mL。MOE和AgNPs对MCF-7细胞系也观察到DNA损伤。然而，MOE和AgNPs对HUVEC细胞系观察到的DNA损伤不显著。本研究结果揭示了生物合成的AgNPs对MCF-7细胞系的细胞毒性和遗传毒性作用。然而，这些AgNPs被认为对正常的HUVEC细胞系是安全的。
