Notermans S, Kozaki S, Dufrenne J, van Schothorst M
Tijdschr Diergeneeskd. 1979 Sep 15;104(18):707-12.
In the winter of 1978-1979, the presence of Clostridium botulinum was studied on a cattle farm, on which botulism caused by feeding the animals contaminated brewers' grains occurred in 1977. Cl. botulinum type B, the cause of mortality among cattle at the time, was detected in grass silage prepared in 1978. This organism was not detectable in a grass silage pit dating from 1977 and made prior to the outbreak of botulism. Investigations showed that proteolytic types of C. botulinum having grass as the substrate may produce large amounts of toxin. The production of toxin in grass silage pits may, however, be prevented by a low pH in conjunction with low water activity. The present study showed that the faeces of cattle were also contaminated with Cl. botulinum type B. The degree of infection ran parallel with the degree of contamination of silage feeding in these cases.
1978 - 1979年冬季,在一个养牛场对肉毒梭菌的存在情况进行了研究。该养牛场在1977年因给动物喂食受污染的啤酒糟而发生了肉毒中毒。在1978年制备的青贮牧草中检测到当时导致牛死亡的B型肉毒梭菌。在1977年肉毒中毒爆发之前建造的一个1977年的青贮牧草窖中未检测到这种微生物。调查表明,以草为底物的蛋白水解型肉毒梭菌可能会产生大量毒素。然而,青贮牧草窖中低pH值与低水分活度相结合可防止毒素产生。本研究表明,牛的粪便也受到B型肉毒梭菌污染。在这些案例中,感染程度与青贮饲料的污染程度平行。