Hughes B J, Forsell J H, Sleight S D, Kuo C, Shull L R
J Anim Sci. 1985 Dec;61(6):1587-603. doi: 10.2527/jas1985.6161587x.
Newborn Holstein bull calves were fed either analytical pentachlorophenol (aPCP) or technical pentachlorophenol (tPCP) for 6 wk to establish and compare the clinical and pathologic manifestations of toxicity. Four groups of three calves/group were each fed either 1 or 10 mg X (kg body weight)-1 X d-1 of either aPCP or tPCP. A fifth group served as control. Dosages of both PCP preparations were normalized to contain equal concentrations of PCP. Toxic effects were observed only at the 10 mg/kg dose in the tPCP-treated calves. These effects included decreased body weight gain, anorexia, decreased serum protein concentration, elevated serum gamma glutamyl transferase, and decreased triiodothyronine (T3) and thyroxine (T4) concentrations. Histologic lesions included cortical atrophy in the thymus and squamous metaplasia and hyperkeratous changes in the Meibomian gland of the eyelid. Thyroid function, which was assessed in vivo by measuring the rate of T3 and T4 production over 4 h after thyrotropin-releasing hormone (TRH)-challenge, was not impaired suggesting an extrathyroidal site of toxic action. Although serum chemistry indicators were suggestive of hepatic injury there were no discernable lesions. Organ weight analyses were inconclusive but there was a tendency toward enlargement of liver, kidneys and thyroid and decreased weight of lungs, spleen and thymus. A toxic effect clearly related to PCP and not its contaminants was depressed active transport of p-aminohippurate measured in kidney slices in vitro. Steady state concentrations of PCP in serum were about 40 and 90 ppm for the 1 and 10 mg/kg groups, respectively. Concentrations of PCP among the major organs were comparable.
给新生荷斯坦公牛犊喂食分析纯五氯苯酚（aPCP）或工业级五氯苯酚（tPCP）6周，以确定并比较毒性的临床和病理表现。将四组（每组三头犊牛）分别喂食1或10 mg×(kg体重)-1×d-1的aPCP或tPCP。第五组作为对照。两种五氯苯酚制剂的剂量均进行了标准化处理，使其五氯苯酚浓度相等。仅在tPCP处理的犊牛中，10 mg/kg剂量时观察到毒性作用。这些作用包括体重增加减少、厌食、血清蛋白浓度降低、血清γ-谷氨酰转移酶升高、三碘甲状腺原氨酸（T3）和甲状腺素（T4）浓度降低。组织学病变包括胸腺皮质萎缩、眼睑睑板腺鳞状化生和角化过度改变。通过测量促甲状腺激素释放激素（TRH）激发后4小时内T3和T4的产生速率来评估体内甲状腺功能，未发现受损，提示毒性作用部位在甲状腺外。尽管血清化学指标提示肝损伤，但未发现明显病变。器官重量分析结果不明确，但肝脏、肾脏和甲状腺有增大趋势，而肺、脾和胸腺重量有减轻趋势。在体外肾切片中测得的对氨基马尿酸主动转运受抑制，这是一种与五氯苯酚明显相关而非其污染物所致的毒性作用。1和10 mg/kg组血清中五氯苯酚的稳态浓度分别约为40和90 ppm。主要器官中五氯苯酚的浓度相当。
