Sooampon Sireerat, Jirojvanichakorn Charit, Ratanapakorn Tanapon, Harnleulomviboon Achawich, Phowan Tharada, Ayuthaya Benjar Issaranggun Na
Department of Pharmacology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Int Dent J. 2025 Apr;75(2):1292-1301. doi: 10.1016/j.identj.2024.09.017. Epub 2024 Oct 5.
While Chlorhexidine mouthwash is widely studied for the treatment of periodontal disease, research on chlorhexidine in the form of dental floss is limited. This study aims to evaluate the effect of chlorhexidine wax-coated dental floss on dental plaque accumulation and gingival bleeding. Additionally, antibacterial activity and cellular toxicity were also investigated in vitro.
Various concentrations of chlorhexidine wax-coated floss (0%, 0.12%, 1%, and 2%) were prepared. The antibacterial activity against Streptococcus mutans was studied using a disc diffusion assay. Cellular toxicity was assessed in L929 cells and human gingival fibroblasts using an MTT assay. To evaluate the effects on plaque accumulation and gingival bleeding, 27 participants were randomly divided into 3 groups: 1) 0% chlorhexidine wax-coated dental floss (control), 2) 0.12% chlorhexidine wax-coated dental floss, and 3) 1% chlorhexidine wax-coated dental floss. All participants were instructed to use the provided dental floss once daily at bedtime for 14 days. Six sites per tooth were evaluated for the Quigley-Hein plaque index and bleeding index (BI) at day 0 (baseline) and day 15. All fully erupted teeth, except the third molars, were examined.
Chlorhexidine-coated floss exhibited antibacterial activity against S. mutans in a dose-dependent manner. In an in-vitro study, a 2% concentration of chlorhexidine in the floss was found to be highly toxic, leading to its exclusion from clinical trials. After 14 days of use, significantly lower levels of BI were observed in the groups using chlorhexidine wax-coated dental floss, compared to the control. Additionally, there was no significant difference in BI between the 0.12% and 1% chlorhexidine wax-coated dental floss groups. However, no significant difference in plaque index was found between the groups using chlorhexidine wax-coated dental floss and the control group.
This study demonstrated the antibacterial and anti-gingivitis properties of chlorhexidine wax-coated dental floss. Our results showed that using chlorhexidine wax-coated dental floss at a concentration as low as 0.12% could significantly reduce gingival bleeding. However, no additional benefit of chlorhexidine wax-coated dental floss on dental plaque accumulation was found.
虽然洗必泰漱口水在牙周疾病治疗方面得到了广泛研究，但关于牙线形式的洗必泰的研究却很有限。本研究旨在评估洗必泰蜡涂层牙线对牙菌斑积聚和牙龈出血的影响。此外，还在体外研究了其抗菌活性和细胞毒性。
制备了不同浓度的洗必泰蜡涂层牙线（0%、0.12%、1%和2%）。使用纸片扩散法研究了其对变形链球菌的抗菌活性。使用MTT法在L929细胞和人牙龈成纤维细胞中评估细胞毒性。为了评估对菌斑积聚和牙龈出血的影响，将27名参与者随机分为3组：1）0%洗必泰蜡涂层牙线（对照组），2）0.12%洗必泰蜡涂层牙线，3）1%洗必泰蜡涂层牙线。所有参与者均被指示每天睡前使用提供的牙线一次，共14天。在第0天（基线）和第15天，对每颗牙齿的6个部位进行Quigley-Hein菌斑指数和出血指数（BI）评估。检查了除第三磨牙外的所有完全萌出的牙齿。
洗必泰涂层牙线对变形链球菌表现出剂量依赖性的抗菌活性。在一项体外研究中，发现牙线中2%浓度的洗必泰具有高毒性，因此将其排除在临床试验之外。使用14天后，与对照组相比，使用洗必泰蜡涂层牙线的组中观察到的BI水平显著降低。此外，0.12%和1%洗必泰蜡涂层牙线组之间的BI没有显著差异。然而，使用洗必泰蜡涂层牙线的组与对照组之间在菌斑指数上没有发现显著差异。
本研究证明了洗必泰蜡涂层牙线的抗菌和抗牙龈炎特性。我们的结果表明，使用低至0.12%浓度的洗必泰蜡涂层牙线可显著减少牙龈出血。然而，未发现洗必泰蜡涂层牙线在牙菌斑积聚方面有额外益处。
