用于鼠伤寒沙门氏菌多色比色检测的靶标抑制型MCOF-Apt-AuNPs自组装体
Target-inhibited MCOF-Apt-AuNPs self-assembly for multicolor colorimetric detection of Salmonella Typhimurium.
作者信息
Li Hang, Xu Hui, Yao Shuo, Wei Shengnan, Liu Yi, Shi Xuening, Zhao Wei, Zhao Chao
机构信息
School of Public Health, Jilin University, Changchun, China.
Department of Nuclear Medicine, China-Japan Union Hospital of Jilin University, Changchun, China.
出版信息
NPJ Sci Food. 2024 Oct 5;8(1):78. doi: 10.1038/s41538-024-00321-7.
Pathogens detection is a crucial measure in the prevention of foodborne diseases. This study developed a novel multicolor colorimetric assay to visually detect Salmonella Typhimurium (S. Typhimurium), by utilizing the etching process of gold nanorods (AuNRs) with TMB. The strategy involved the construction of nanozyme by assembling magnetic covalent organic framework (MCOF) with aptamer-conjugated AuNPs (Apt-AuNPs), exhibiting remarkable peroxidase-like activity to catalyze the oxidation of TMB/HO and inducing the etching of AuNRs. The presence of S. Typhimurium could inhibit this process, resulting in the generation of vivid colors. The multicolor colorimetric assay could specifically determine S. Typhimurium from 10 to 10 CFU mL in 60 min with visual detection limit of 10 CFU mL, and instrumental detection limit of 2.3 CFU mL. Moreover, detecting S. Typhimurium in chicken, milk, pork and lettuce samples has shown promise in practical applications.
病原体检测是预防食源性疾病的一项关键措施。本研究开发了一种新型多色比色法，通过利用金纳米棒（AuNRs）与TMB的蚀刻过程来目视检测鼠伤寒沙门氏菌（S. Typhimurium）。该策略涉及通过将磁性共价有机框架（MCOF）与适配体共轭金纳米粒子（Apt-AuNPs）组装来构建纳米酶，其表现出显著的过氧化物酶样活性以催化TMB/H₂O₂的氧化并诱导AuNRs的蚀刻。鼠伤寒沙门氏菌的存在会抑制这一过程，从而产生鲜明的颜色变化。这种多色比色法能够在60分钟内从10³至10⁶CFU/mL的范围内特异性地测定鼠伤寒沙门氏菌，目视检测限为10³CFU/mL，仪器检测限为2.3CFU/mL。此外，在鸡肉、牛奶、猪肉和生菜样品中检测鼠伤寒沙门氏菌已显示出在实际应用中的前景。
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